The wound dressing can temporarily replace the skin and play a protective role in the process of wound healing, preventing wound infection and inflammation, and providing a favorable environment for wound healing. In this study, a mixture of collagen and chitosan was lyophilized to be the host material of the sponge. This sponge was soaked into 1-ethyl-(dimethylaminopropyl) carbodiimide/N-hydroxy sulfosuccinimide cross-linking solution containing heparin and experienced secondary lyophilization to prepare the heparinized sponge (CT-CL/Hp). The surface morphology and structural characterization of the sponge was characterized by scanning electron microscope and Fourier transform infrared spectrometer, respectively. Relatively favorable water absorption capability were observed by measuring the physical properties. Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro. The sustained-release property of heparin from the sponges was measured using Alcian Blue assay. Experiments in vitro and in vivo showed that the sponges had satisfactory biocompatibility and lower sensitization. Moreover, the effect of sponge on early stages of wound healing was evaluated by guinea pigs wound healing models. Analysis of wound healing rates and histological examination showed satisfactory results. CT-CL/Hp enhanced expression of growth factors, particularly VEGF and EGF at day 7. These results demonstrated that CT-CL/Hp–treated sponges benefit to wound skin healing and regeneration.

中文翻译：

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基于胶原蛋白和壳聚糖的用于伤口愈合的肝素海绵的制备及评价

伤口敷料可以暂时代替皮肤，在伤口愈合过程中起到保护作用，防止伤口感染和炎症，为伤口愈合提供良好的环境。在这项研究中，胶原蛋白和壳聚糖的混合物被冻干作为海绵的主体材料。将该海绵浸入含有肝素的1-乙基-(二甲氨基丙基)碳二亚胺/N-羟基磺基琥珀酰亚胺交联溶液中并进行二次冻干以制备肝素化海绵(CT-CL/Hp)。海绵的表面形貌和结构表征分别通过扫描电子显微镜和傅里叶变换红外光谱仪进行表征。通过测量物理性能观察到相对良好的吸水能力。通过体外抗菌效果分析观察到对各种细菌的抗菌性能和微生物分离性能令人满意。肝素从海绵中的缓释特性使用爱新蓝测定法测量。体外和体内实验表明，该海绵具有令人满意的生物相容性和较低的致敏性。此外，通过豚鼠伤口愈合模型评估了海绵对伤口愈合早期阶段的影响。伤口愈合率分析和组织学检查结果令人满意。CT-CL/Hp 在第 7 天增强了生长因子的表达，特别是 VEGF 和 EGF。这些结果表明 CT-CL/Hp 处理的海绵有利于伤口皮肤的愈合和再生。