Abstract

In this study, we investigated the role of XIST in the development of diabetic nephropathy (DN) and further explored its underlying mechanism. qRT-PCR was used to examine the level of XIST in serum of DN patients. ELISA, MTT, and flow cytometry were used to investigate the effect of XIST on biological functions of human mesangial cells (HMCs) treated with high glucose. The recovery experiments were used to explore the potential mechanism. The result showed XIST expression was elevated significantly in serums of DN patients. XIST silencing alleviated the induction of high glucose in biological behaviour of HMCs. Besides, miR-485 inhibitor revised the suppression by si-XIST in biological behaviour of high glucose induced HMCs. Furthermore, PSMB8 mimic relieved the inhibition of si-XIST in biological behaviour of high glucose induced HMCs. In short, XIST silencing could alleviate biological process and inflammation of HMCs treated with high glucose by sponging miR-485.

摘要

在这项研究中，我们调查了 XIST 在糖尿病肾病 (DN) 发展中的作用，并进一步探讨了其潜在机制。qRT-PCR用于检测DN患者血清中XIST的水平。ELISA、MTT 和流式细胞术用于研究 XIST 对高糖处理的人系膜细胞 (HMC) 生物学功能的影响。回收实验用于探索潜在机制。结果显示，DN患者血清中XIST表达显着升高。XIST 沉默减轻了 HMC 生物学行为中高糖的诱导。此外，miR-485抑制剂修正了si-XIST对高糖诱导的HMCs生物学行为的抑制作用。此外，PSMB8 模拟物减轻了 si-XIST 在高糖诱导的 HMC 生物学行为中的抑制作用。简而言之，