Abstract

From the ethyl acetate extract of the stems of Miliusa velutina, seven compounds (1–7) were isolated, including two new compounds such as mivelutina A acid (1), mivelutina B acid (2) and one known compound mivelutina B methyl ester (3). For this NMR data were not known previously. Their relative structures were elucidated based on NMR spectroscopic analysis. The absolute configuartions were determined based on DFT calculations of ¹³C chemical shifts. All of the seven compounds were screened for their in vitro cytotoxic activities against HepG2 cell line using the SRB assay. Epoxyconiferyl alcohol (7) showed the highest potential for the cytotoxicity of cancer cell lines HepG2 with the IC₅₀ values of 95.94 μg/mL (527 μM).

摘要

从的乙酸乙酯提取物茎野独绒毛，七种化合物（1 - 7）进行分离，其中包括两个新的化合物，例如mivelutina A酸（1），mivelutina乙酸（2）和一种已知化合物mivelutina乙酸甲酯（3）。对于这个 NMR 数据，以前是未知的。基于NMR光谱分析阐明了它们的相对结构。绝对构型是基于¹³ C 化学位移的DFT 计算确定的。使用 SRB 测定法筛选所有七种化合物对 HepG2 细胞系的体外细胞毒活性。环氧松柏醇 ( 7) 显示出对癌细胞系 HepG2 的细胞毒性的最高潜力，IC ₅₀值为 95.94 μg/mL (527 μM)。