ABSTRACT In the present work, the microencapsulation conditions by spray drying of the biocontrol agent Meyerozyma guilliermondii using the multi-response surface methodology (MRSM) were optimised. The concentration of sodium alginate and soy protein isolate, encapsulating agents, and the dryer inlet air temperature were evaluated on the percentage of yeast viability, release rate, and percentage of mycelial growth inhibition of Colletotrichum gloeosporioides isolated from mango. The optimum conditions obtained by MRSM were 0.06% sodium alginate (w/v), 3.8% soy protein isolate (w/v), and dryer inlet air temperature of 113°C. The validation of the optimal conditions allowed to obtain values of 97.8 ± 1.53% viability, the release rate of 0.124 ± 0.04 Log CFU g·h−1 and 86.4 ± 1.1% of mycelial growth inhibition. A hydratable powder formulation was produced with the microencapsulated biocontrol agent, which is considered an effective, integrated, and eco-friendly alternative to manage the control of anthracnose disease caused by C. gloeosporioides on mango fruit.

中文翻译：

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以海藻酸钠和大豆分离蛋白为壁材的喷雾干燥微囊化Meyerozyma guilliermondii：芒果炭疽病的生物防治制剂

摘要 在目前的工作中，优化了使用多响应表面方法 (MRSM) 对生物防治剂 Meyerozyma guilliermondii 进行喷雾干燥的微囊化条件。海藻酸钠和大豆分离蛋白、包封剂和干燥器入口空气温度的浓度对酵母活力的百分比、释放率和从芒果中分离的胶孢炭疽菌的菌丝生长抑制百分比进行了评估。MRSM 获得的最佳条件为 0.06% 海藻酸钠 (w/v)、3.8% 大豆分离蛋白 (w/v) 和干燥机入口空气温度 113°C。最佳条件的验证允许获得 97.8 ± 1.53% 的活力值、0.124 ± 0.04 Log CFU g·h-1 的释放率和 86.4 ± 1.1% 的菌丝体生长抑制。