ABSTRACT

Diazinon (DZN) is an organophosphate insecticide that affects the liver adversely. Ginger exhibits antioxidant properties. We investigated the hepatoprotective effects of an ethanolic extract of ginger root on DZN induced hepatotoxicity. We measured total phenolics and flavonoids in the hydroalcoholic extract. We used Wistar rats divided into four groups: control, 100 mg/kg/day ginger by gavage, 10 mg/kg/day DZN intraperitoneally, and ginger + DZN group treated with ginger 1 h before DZN. All treatments were for 30 consecutive days. One day after the last treatment, we evaluated oxidative stress parameters, serum biochemistry, histopathology and immunohistochemistry. The ginger extract contained 101.33 ± 2.73 mg total flavonoid and 237.9 ± 3 mg total phenolic content/g dry ginger plant roots. We found that DZN increased oxidative stress significantly. Histopathology of the liver tissue was consistent with increased AST, ALT, and ALP. Ginger extract treatment reduced oxidative stress and improved histopathology. DZN increased caspase-3 immunoreactivity and ginger extract reduced it. Ginger extract exhibited hepatoprotective effects against DZN induced hepatic injury owing to its antioxidant and anti-apoptotic activity

摘要

二嗪农 (DZN) 是一种对肝脏有不利影响的有机磷杀虫剂。生姜具有抗氧化特性。我们研究了姜根乙醇提取物对 DZN 诱导的肝毒性的保肝作用。我们测量了水醇提取物中的总酚类和类黄酮。我们使用分为四组的 Wistar 大鼠：对照组，100 毫克/公斤/天姜灌胃，10 毫克/公斤/天 DZN 腹腔注射，姜 + DZN 组在 DZN 前 1 小时用姜处理。所有处理连续30天。最后一次治疗后一天，我们评估了氧化应激参数、血清生物化学、组织病理学和免疫组织化学。生姜提取物含有 101.33 ± 2.73 mg 总黄酮和 237.9 ± 3 mg 总酚含量/g 干姜植物根。我们发现 DZN 显着增加了氧化应激。肝组织的组织病理学与 AST、ALT 和 ALP 升高一致。生姜提取物治疗可减少氧化应激并改善组织病理学。DZN 增加了 caspase-3 的免疫反应性，而生姜提取物则降低了它。生姜提取物由于其抗氧化和抗凋亡活性而对 DZN 诱导的肝损伤具有保肝作用