Lettuce (Lactuca sativa), cultivated mainly for its edible leaves and stems, is an important vegetable crop worldwide. Genomes of cultivated lettuce (L. sativa cv. Salinas) and its wild relative L. serriola accession US96UC23 are sequenced, but a clear understanding of the genetic basis for divergence in phenotypes of the two species is lacking. Tandem mass tag (TMT) based mass spectrometry is used to quantitatively compare protein levels between these two species. Four‐day old seedlings is transplanted into 500 mL pots filled with soil. Plants are grown for 8 weeks under 250 µmol m⁻² sec⁻¹ continuous light, 20 °C and relative humidity between 50–70%. Leaf discs (1 cm diameter) from three individuals per biological replicate are analyzed. A total of 3000 proteins are identified, of which the levels of 650 are significantly different between ‘Salinas’ and US96UC23. Pathway analysis indicated a higher flux of carbon in ‘Salinas’ than US96UC23. Many essential metabolic pathways such as tetrapyrrole metabolism and fatty acid biosynthesis are upregulated in ‘Salinas’ compared with US96UC23. This study provides a reference proteome for researchers interested in understanding lettuce biology and improving traits for cultivation.

中文翻译：

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蛋白质组学分析表明，在莴苣 cv. 中，更多的蛋白质参与了主要代谢途径。Salinas 比 Lactuca serriola 登记号 US96UC23。

莴苣（Lactuca sativa）主要以其可食用的叶和茎种植，是世界上重要的蔬菜作物。已对栽培生菜 ( L. sativa cv. Salinas) 及其野生近缘L. serriola登录 US96UC23 的基因组进行了测序，但缺乏对这两个物种表型差异的遗传基础的清楚了解。基于串联质量标签 (TMT) 的质谱法用于定量比较这两个物种之间的蛋白质水平。将 4 天大的幼苗移植到装满土壤的 500 毫升盆中。植物在 250 µmol m ^-2 sec ^-1下生长 8 周连续光照，20 °C，相对湿度在 50-70% 之间。分析了每个生物重复来自三个个体的叶盘（直径 1 厘米）。总共鉴定了 3000 种蛋白质，其中 650 种水平在“Salinas”和 US96UC23 之间有显着差异。通路分析表明，'Salinas' 中的碳通量高于 US96UC23。与 US96UC23 相比，'Salinas' 中的许多基本代谢途径如四吡咯代谢和脂肪酸生物合成上调。这项研究为有兴趣了解生菜生物学和改善栽培性状的研究人员提供了参考蛋白质组。