Abstract 1-Methylcyclopropene (1-MCP) has been widely used to manipulate fruit ripening. However, inappropriate treatment with 1-MCP may cause ripening disorders. In this study, we observed that the appropriate concentration of 1-MCP (400 nl L−1, 6 h) (1-MCP400) significantly delayed the ripening of Fenjiao banana. However, a high concentration of 1-MCP (3200 nl L−1, 6 h) (1-MCP3200) resulted in abnormal Fenjiao banana that ripened with softened fruit that had a green peel. An RNA sequencing analysis showed that a large number of differentially expressed genes (DEGs) in the fruit peel and pulp were screened out from fruit that were ripened as controls. A KEGG analysis revealed that the metabolic pathways of plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, photosynthesis and biosynthesis of amino acids were significantly enriched during fruit ripening. The fruit transcript level of fruit was markedly altered by 1-MCP treatment. Large numbers of DEGs were also detected between high and appropriate concentrations of 1-MCP in the peel and pulp. A comprehensive functional enrichment analysis showed that most of the DEGs involved in photosynthesis, cysteine and methionine metabolism, phenylpropanoid biosynthesis, amino sugar and nucleotide sugar metabolism, plant hormone signal transduction, starch and sucrose metabolism were enriched. RT-qPCR verified the RNA-Seq results, which indicated that 1-MCP3200 severely repressed the expression of genes involved in ethylene (MaACS1, MaACO1, MaETR2-like, MaERF003, MaERF012 and MaERF113), auxin (MaARF19-like, MaSAUR71-like and MaSAUR72-like) and abscisic acid (MaPYL3-like and MaABI5-like) signaling pathways, chlorophyll and cell wall degradation, and starch and sucrose metabolism but induced the expression of genes in lignin synthesis. These genes were consistently expressed in the pulp and peel following control and 1-MCP400 treatment, but their expression was inconsistent following 1-MCP3200 treatment, which may result in the failure of the peel to turn yellow in the 1-MCP3200 group.

中文翻译：

---

转录组学分析揭示了调节 1-MCP 处理引起的香蕉果皮（粉胶）保持绿色障碍的关键因素

摘要 1-甲基环丙烯（1-MCP）已被广泛用于调控果实成熟。然而，1-MCP 处理不当可能会导致催熟障碍。在本研究中，我们观察到适当浓度的 1-MCP (400 nl L−1, 6 h) (1-MCP400) 显着延迟了粉蕉香蕉的成熟。然而，高浓度的 1-MCP (3200 nl L−1, 6 h) (1-MCP3200) 导致异常的粉蕉成熟，果实变软，果皮呈绿色。RNA测序分析表明，从作为对照的成熟果实中筛选出大量果皮和果肉中的差异表达基因（DEGs）。KEGG 分析表明，植物激素信号转导、淀粉和蔗糖代谢、苯丙烷生物合成、果实成熟期间氨基酸的光合作用和生物合成显着丰富。1-MCP 处理显着改变了果实的果实转录水平。在果皮和果肉中高浓度和适当浓度的 1-MCP 之间也检测到大量 DEG。综合功能富集分析表明，大部分参与光合作用、半胱氨酸和蛋氨酸代谢、苯丙烷生物合成、氨基糖和核苷酸糖代谢、植物激素信号转导、淀粉和蔗糖代谢的DEGs被富集。RT-qPCR验证了RNA-Seq结果，表明1-MCP3200严重抑制乙烯（MaACS1、MaACO1、MaETR2-like、MaERF003、MaERF012和MaERF113）、生长素（MaARF19-like、MaSAUR71 样和 MaSAUR72 样）和脱落酸（MaPYL3 样和 MaABI5 样）信号通路、叶绿素和细胞壁降解以及淀粉和蔗糖代谢，但诱导木质素合成中基因的表达。这些基因在对照和1-MCP400处理后的果肉和果皮中一致表达，但在1-MCP3200处理后它们的表达不一致，这可能导致1-MCP3200组果皮未能变黄。