A rapid and sensitive method utilizing flash evaporation-gas chromatography/mass spectrometry (FE-GC/MS) has been developed. The method is applicable to determine ketamine (KET), methamphetamine (MAMP) and 3,4-methylenedioxymethamphetamine (MDMA) in human hair. Cut and weighted hair (0.30 mg) was heated at the flash evaporation temperature of 350 °C. KET, MAMP and MDMA were released into a capillary column for GC/MS analysis and produced fragment ions in SIM mode. Validation of the method included evaluation of linearity, sensitivity, accuracy, precision and repeatability. Linearity ranged from 2 to 300 ng/mg for KET in hair and 2 to 200 ng/mg for MAMP and MDMA in hair with the correlation coefficients all greater than 0.998. Limits of detection were 0.7 ng/mg and limits of quantification were 2.0 ng/mg of hair for KET, MAMP and MDMA. The precision ranged from 1.57% to 7.75% for KET, 1.49% to 7.10% for MAMP and 1.84% to 8.31% for MDMA. The recovery ranged from 102.1% to 110.9% for KET, 99.3% to 108.0% for MAMP and 89.5% to 112.6% for MDMA. Six authentic hair samples from known drug abusers and three drug-free hair samples from volunteers who had never used drugs were successfully analyzed. Compared with traditional time-consuming and hair-comsuming pretreatment method, FE-GC/MS was a faster, simpler and low sample consumption method for the determination of KET, MAMP and MDMA in human hair.

已经开发出一种快速灵敏的方法，利用闪蒸-气相色谱/质谱（FE-GC / MS）。该方法适用于测定人发中的氯胺酮（KET），甲基苯丙胺（MAMP）和3,4-亚甲二氧基甲基苯丙胺（MDMA）。剪下并称重的头发（0.30 mg）在闪蒸温度350°C下加热。KET，MAMP和MDMA被释放到毛细管柱中进行GC / MS分析，并以SIM模式产生碎片离子。该方法的验证包括评估线性，灵敏度，准确性，精密度和可重复性。头发中KET的线性范围为2到300 ng / mg，头发中MAMP和MDMA的线性范围为2到200 ng / mg，相关系数都大于0.998。对于KET，MAMP和MDMA，头发的检出限为0.7 ng / mg，定量限为2.0 ng / mg。KET的精度范围为1.57％至7.75％，MAMP的精度范围为1.49％至7.10％，MDMA的精度范围为1.84％至8.31％。KET的回收率从102.1％到110.9％，MAMP的回收率从99.3％到108.0％，MDMA的回收率从89.5％到112.6％。成功分析了六个已知吸毒者的真实头发样本和三个从未使用过药物的志愿者的无毒品头发样本。与传统的费时且耗费头发的预处理方法相比，FE-GC / MS是一种用于测定人发中KET，MAMP和MDMA的更快，更简单且样品消耗更少的方法。成功分析了六个已知吸毒者的真实头发样本和三个从未使用过药物的志愿者的无毒品头发样本。与传统的费时且耗费头发的预处理方法相比，FE-GC / MS是一种用于测定人发中KET，MAMP和MDMA的更快，更简单且样品消耗更少的方法。成功分析了六个已知吸毒者的真实头发样本和三个从未使用过药物的志愿者的无毒品头发样本。与传统的费时且耗费头发的预处理方法相比，FE-GC / MS是一种用于测定人发中KET，MAMP和MDMA的更快，更简单且样品消耗更少的方法。