Gene ( IF 3.5 ) Pub Date : 2020-07-16 , DOI: 10.1016/j.gene.2020.144952 Xiaoxu Ge 1 , Liuqing Xi 1 , Qianqian Wang 1 , Huihua Li 1 , Lili Xia 1 , Zhen Cang 1 , Wenfang Peng 1 , Shan Huang 1
Diabetic nephropathy (DN) as one of the most frequent microvascular complications of diabetic patients causes chronic renal failure and end-stage renal disease. Noncoding RNAs including circular RNAs (circRNAs) and micro RNAs (miRNAs) were widely reported to play a critical role in numerous human diseases including DN. This research was designed to investigate the role of circ_0000064 in diabetic nephropathy progression. The results showed that circ_0000064 significantly promoted mesangial cells proliferation and aggravated fibrosis in DN. In the subsequent mechanism investigation, we found that circ_0000064 was involved in this process by targeting micro RNA miR-143. The inhibition of miR-143 significantly reverses the effect of circ_0000064 silencing on DN. In conclusion, we demonstrated the regulatory role of circ_0000064 in DN and clarified that circ_0000064 play a role in DN via targeting miR-143. Circ_0000064 and miR-143 also showed the potential as a biomarker for DN.
中文翻译:
环状RNA Circ_0000064通过miR-143促进糖尿病肾病中系膜细胞的增殖和纤维化。
糖尿病肾病(DN)是糖尿病患者最常见的微血管并发症之一,可导致慢性肾衰竭和终末期肾脏疾病。据广泛报道,包括环状RNA(circRNA)和微小RNA(miRNA)在内的非编码RNA在包括DN在内的许多人类疾病中起着至关重要的作用。这项研究旨在调查circ_0000064在糖尿病肾病进展中的作用。结果表明,circ_0000064显着促进了DN中系膜细胞的增殖并加剧了纤维化。在随后的机理研究中,我们发现circ_0000064通过靶向micro RNA miR-143参与了这一过程。miR-143的抑制作用显着逆转了circ_0000064沉默对DN的影响。结论,我们证明了circ_0000064在DN中的调节作用,并阐明circ_0000064通过靶向miR-143在DN中发挥作用。Circ_0000064和miR-143也显示了作为DN生物标志物的潜力。