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The Influence of Effectors of the Ca 2+ Signaling System and Oligosaccharin OSRG on IAA-Induced Formation of Adventitious Roots on Explants of Buckwheat Hypocotyls
Russian Journal of Plant Physiology ( IF 1.4 ) Pub Date : 2020-07-16 , DOI: 10.1134/s1021443720040111
I. A. Larskaya , O. V. Gorshkov , O. I. Trofimova , T. A. Gorshkova

Abstract

The interplay between different signaling pathways that occurs during the development of a root system is investigated by considering, by way of example, the role of effectors of the Ca2+ signaling system and a rhizogenesis-stimulating oligosaccharin (OSRG) on the indoleacetic acid (IAA)-induced formation of adventitious roots. By identifying the sensitivity of different stages of rhizogenesis in cultivated buckwheat hypocotyls (Fagopyrum esculentum Moench) to IAA, Ca2+, and the oligosaccharin, it is shown that both Ca2+ and OSRG affect the root formation processes at its initial stages, preceding the effect of the hormone. Auxin can be added to the medium with some delay but no later than 1 h after the explant cultivation was started; while Ca2+ must be present in the medium from the very beginning of cultivation process. The number of IAA-induced adventitious roots on segments of buckwheat hypocotyls decreases in the presence of Ca2+ channel blockers (verapamil and diltiazem), compounds affecting Ca2+-release from intracellular storages (ruthenium red, neomycin), and calmodulin-antagonists (chlorpromazine and fluphenazine). Using calcium channel blocker (diltiazem), it is shown that the effect caused by the oligosaccharin does not depend on the functioning of potential-dependent channels of plasma membrane. The analysis of transcriptome profiling data for the cultivated explants identifies the expression of 149 genes (TGR > 5) relevant to Ca2+ signaling in investigated specimens. The expression profile of these genes changes markedly during IAA-induced root formation, while the addition of OSRG affecting the expression of only few of those.



中文翻译:

Ca 2+信号系统和寡糖OSRG的效应物对IAA诱导的荞麦胚轴外植体不定根形成的影响

摘要

例如,通过考虑Ca 2+信号系统的效应子和吲哚乙酸上的发根促进寡糖(OSRG)的作用,研究了在根系统发育过程中发生的不同信号通路之间的相互作用。IAA)诱导不定根的形成。通过鉴定培养的荞麦下胚轴(Fagopyrum esculentum Moench)根际形成的不同阶段对IAA,Ca 2+和寡糖精的敏感性,表明Ca 2+在激素作用之前,OSRG和OSRG在其初始阶段影响根形成过程。生长素可以在外植体培养开始后不迟于1 h的时间内添加到培养基中。而Ca 2+必须从培养过程的一开始就存在于培养基中。在存在Ca 2+通道阻滞剂(维拉帕米和地尔硫卓)的情况下,荞麦下胚轴部分上IAA诱导的不定根的数量减少,而Ca 2+通道阻滞剂会影响Ca 2+释放从细胞内存储(钌红,新霉素)和钙调蛋白拮抗剂(氯丙嗪和氟奋乃静)。使用钙通道阻滞剂(地尔硫卓)显示,寡糖精所引起的作用不依赖于质膜电位依赖性通道的功能。对培养的外植体的转录组谱分析数据的分析确定了在研究标本中与Ca 2+信号传导相关的149个基因(TGR> 5)的表达。这些基因的表达谱在IAA诱导的根形成过程中发生了显着变化,而OSRG的添加仅影响其中少数的表达。

更新日期:2020-07-16
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