Abstract

The present research aimed to investigate the effect of ethylene on the physiological and molecular responses of the cut roses, Rosa hybrida L. Two cut rose cultivars (‘Amada +’ and ‘Brillante’) with different ethylene sensitivity were treated by 10 ppm exogenous ethylene for 24 h under identical vase life room conditions at partially open stage. The cultivars were then examined at both partially and fully open stage by gas chromatography, UV/Vis spectroscopy, and qRT-PCR. The cultivar with high sensitivity had lower total protein contents and showed higher ethylene production and antioxidant activity. Either exogenously applied ethylene or endogenously produced one resulted in increased antioxidant activity and decreased total protein contents. This observation is possibly because of enhanced free radical generation and protease activation with ethylene-induced flower opening and senescence. The qRT-PCR analysis showed a significant increase in the expression of ethylene signal transduction genes (RhETR1,3 and RhCTR1,2) of the treated cultivars with high sensitivity at commercial stage. However, there was a significant up-regulation of the RhETR1,3 genes in the cultivars with low sensitivity at fully-open stage. The ethylene induced responses were found highly dependent on the cultivar ability in both ethylene synthesis and perception.

中文翻译：

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乙烯诱导切花月季开放花，受体基因表达和抗氧化防御反应

摘要

本研究旨在研究乙烯对切花玫瑰，蔷薇花的生理和分子响应的影响。L.在相同的花瓶寿命室内条件下，在部分开放阶段，用10 ppm外源乙烯处理两个乙烯敏感性不同的切玫瑰品种（“ Amada +”和“ Brillante”）。然后通过气相色谱，UV / Vis光谱和qRT-PCR在部分和完全开放阶段对这些品种进行检查。具有高敏感性的品种总蛋白含量较低，并具有较高的乙烯产量和抗氧化活性。外源施加的乙烯或内源产生的乙烯导致抗氧化剂活性增加和总蛋白质含量降低。该观察结果可能是由于自由基的产生和蛋白酶的活化以及乙烯诱导的开花和衰老所致。商业化处理后的品种的RhETR1,3和RhCTR1,2）具有很高的敏感性。然而，在全开放阶段，低敏感性品种的RhETR1,3基因有明显的上调。发现乙烯诱导的应答高度依赖于乙烯合成和感知中的品种能力。