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Comparative proteomic analysis of drought and high temperature response in roots of two potato cultivars
Plant Growth Regulation ( IF 4.2 ) Pub Date : 2020-07-16 , DOI: 10.1007/s10725-020-00643-y
Dominika Boguszewska-Mańkowska , Marta Gietler , Małgorzata Nykiel

A comparative analysis of drought and high temperature responsive proteins, which means to provide insight into the molecular mechanism of potato stress tolerance. In the presented study, two potato cultivars, differing in dehydration tolerance, were compared. An analysis of their morphological, physiological and root proteome related traits proved that, although water shortage, as well as high temperatures cause the dehydration of plants, the response to those stresses at the proteome level was significantly different. LC–MS/MS protein identification showed that in roots of the sensitive cultivar, in response to drought, most changes concern increased abundance of defence- and detoxification-related proteins, while in tolerant plants, significant changes in abundance of energy and carbohydrate metabolism related proteins were observed (data are available via ProteomeXchange with identifier PXD020259). Moreover, in response to high temperatures, in the sensitive cultivar, decreased abundance of proteins involved in cell energetic metabolism was detected, while in the tolerant cultivar, the majority of proteins from this group was abundant. It can be suggested that such comparative proteome analysis indicates the fine tuning metabolism as a major factor of stress tolerance of potato plants.

中文翻译:

两个马铃薯品种根系干旱和高温响应的蛋白质组学比较分析

干旱和高温响应蛋白的比较分析,这意味着深入了解马铃薯胁迫耐受的分子机制。在本研究中,对脱水耐受性不同的两个马铃薯品种进行了比较。对其形态、生理和根蛋白质组相关性状的分析证明,虽然缺水和高温会导致植物脱水,但在蛋白质组水平上对这些胁迫的反应却存在显着差异。LC-MS/MS 蛋白质鉴定表明,在敏感品种的根中,响应干旱,大多数变化与防御和解毒相关蛋白质的丰度增加有关,而在耐受植物中,观察到能量和碳水化合物代谢相关蛋白质丰度的显着变化(数据可通过 ProteomeXchange 获得,标识符为 PXD020259)。此外,响应高温,在敏感品种中,检测到参与细胞能量代谢的蛋白质丰度降低,而在耐受品种中,该组的大部分蛋白质含量丰富。可以认为,这种比较蛋白质组分析表明微调代谢是马铃薯植物胁迫耐受性的主要因素。该组中的大多数蛋白质含量丰富。可以认为,这种比较蛋白质组分析表明微调代谢是马铃薯植物胁迫耐受性的主要因素。该组中的大多数蛋白质含量丰富。可以认为,这种比较蛋白质组分析表明微调代谢是马铃薯植物胁迫耐受性的主要因素。
更新日期:2020-07-16
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