Improving the reproducibility of biomedical research is a major challenge. Transparent and accurate reporting is vital to this process; it allows readers to assess the reliability of the findings and repeat or build upon the work of other researchers. The ARRIVE guidelines (Animal Research: Reporting In Vivo Experiments) were developed in 2010 to help authors and journals identify the minimum information necessary to report in publications describing in vivo experiments. Despite widespread endorsement by the scientific community, the impact of ARRIVE on the transparency of reporting in animal research publications has been limited. We have revised the ARRIVE guidelines to update them and facilitate their use in practice. The revised guidelines are published alongside this paper. This explanation and elaboration document was developed as part of the revision. It provides further information about each of the 21 items in ARRIVE 2.0, including the rationale and supporting evidence for their inclusion in the guidelines, elaboration of details to report, and examples of good reporting from the published literature. This document also covers advice and best practice in the design and conduct of animal studies to support researchers in improving standards from the start of the experimental design process through to publication.

提高生物医学研究的可重复性是一项重大挑战。透明和准确的报告对于这一过程至关重要；它允许读者评估研究结果的可靠性，并重复或借鉴其他研究人员的工作。ARRIVE 指南（动物研究：报告体内实验）于 2010 年制定，旨在帮助作者和期刊确定在描述体内实验的出版物中报告所需的最少信息。尽管得到了科学界的广泛认可，但 ARRIVE 对动物研究出版物报告透明度的影响仍然有限。我们修订了 ARRIVE 指南以更新它们并促进其在实践中的使用。修订后的指南与本文一起发布。本解释和阐述文件是作为修订的一部分而制定的。它提供了有关 ARRIVE 2.0 中 21 个项目中每一个项目的进一步信息，包括将其纳入指南的基本原理和支持证据、报告细节的阐述以及已发表文献中的良好报告示例。本文件还涵盖了动物研究设计和实施方面的建议和最佳实践，以支持研究人员从实验设计过程开始到发表的整个过程中提高标准。