当前位置: X-MOL 学术J. Econ. Entomol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Mortality of Potato Psyllid (Hemiptera: Triozidae) on Host Clippings Inoculated With Ergot Alkaloids
Journal of Economic Entomology ( IF 2.2 ) Pub Date : 2020-07-11 , DOI: 10.1093/jee/toaa144
Navneet Kaur 1 , W Rodney Cooper 2 , Jennifer M Duringer 1 , Arash Rashed 3 , Ismael E Badillo-Vargas 4 , Gabriela Esparza-Díaz 4 , David R Horton 2
Affiliation  

Abstract Our previous study provided correlative evidence that morning glory species harboring endophytic fungi (Periglandula) are resistant to potato psyllid [Bactericera cockerelli (Šulc)], whereas species free of fungi often allowed psyllid development. In this study, we manipulated levels of ergot alkaloids in host tissues by inoculating clippings from potato plants with extracts from morning glories that harbor Periglandula [Ipomoea leptophylla Torrey, Ipomoea imperati (Vahl) Grisebach, Ipomoea tricolor Cavanilles, Ipomoea pandurata (L.) G. F. Meyer, and Turbina corymbosa (L.)] and one species (Ipomoea alba L.) that does not harbor the endophyte. Ergot alkaloids (clavines, lysergic acid amides, and ergopeptines) were detected in potato clippings, thus confirming that leaves had taken up compounds from solutions of crude extracts. Psyllid mortality rates on inoculated clippings ranged between 53 and 93% in treatments producing biochemically detectable levels of alkaloids, when compared with 15% mortality in water controls or the alkaloid-free I. alba. We then tested synthetic analogs from each of the three alkaloid classes that had been detected in the crude extracts. Each compound was assayed by inoculating clippings of two host species (potato and tomato) at increasing concentrations (0, 1, 10, and 100 µg/ml in solution). Psyllids exhibited a large and significant increase in mortality rate beginning at the lowest two concentrations, indicating that even very small quantities of these chemicals led to mortality. Feeding by nymphs on artificial diets containing synthetic compounds resulted in 100% mortality within 48 h, irrespective of compound. Further testing of ergot alkaloids to characterize the mode of action that leads to psyllid mortality is warranted.

中文翻译:

接种麦角生物碱的宿主剪下的马铃薯木虱(半翅目:Triozidae)的死亡率

摘要 我们之前的研究提供了相关证据,表明携带内生真菌 (Periglandula) 的牵牛花物种对马铃薯木虱 [Bactericera cockerelli (Šulc)] 具有抗性,而不含真菌的物种通常允许木虱发育。在这项研究中,我们通过用牵牛花提取物接种马铃薯植物的剪报来控制宿主组织中麦角生物碱的水平 [Ipomoea leptophylla Torrey、Ipomoea imperati (Vahl) Grisebach、Ipomoea tricolor Cavanilles、Ipomoea pandurata (L.) Meyer 和 Turbina corymbosa (L.)] 和一种没有内生菌的物种 (Ipomoea alba L.)。在马铃薯剪屑中检测到麦角生物碱(clavines、麦角酸酰胺和麦角肽碱),从而证实叶子已经吸收了粗提取物溶液中的化合物。在产生生物化学可检测水平的生物碱的处理中,接种剪报的木虱死亡率介于 53% 和 93% 之间,而在水对照或不含生物碱的白杨木虱中死亡率为 15%。然后,我们测试了粗提取物中检测到的三种生物碱类中每一种的合成类似物。通过以递增浓度(溶液中的 0、1、10 和 100 µg/ml)接种两种宿主物种(马铃薯和番茄)的剪报来测定每种化合物。从最低的两个浓度开始,木虱的死亡率显着增加,表明即使非常少量的这些化学物质也会导致死亡率。若虫以含有合成化合物的人工饲料喂养,在 48 小时内导致 100% 的死亡率,与化合物无关。
更新日期:2020-07-11
down
wechat
bug