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Structural Investigation of Epididymal Microvasculature and Its Relation to Telocytes and Immune Cells in Camel
Microscopy and Microanalysis ( IF 2.8 ) Pub Date : 2020-07-15 , DOI: 10.1017/s1431927620001786
Manal T Hussein 1 , Fatma M Abdel-Maksoud 1
Affiliation  

The vascular and perivascular cells, including telocytes (TCs) and immune cells, play an important role in male fertility. The current study intended to describe in detail the microvascular structures harboring special regulatory devices in addition to the interstitial cellular components of the one-humped camel epididymis. The samples were collected from 10 clinically healthy mature camels (Camelus dromedarius). The distribution and characteristics of TCs, peripheral blood vessels of the epididymis, and immune cells were investigated using the light, immunohistochemistry, immunofluorescence, and transmission electron microscopy analyses. Frequent occlusive or throttle arterioles were demonstrated in the epididymal interstitium and their tunica media consisted of glomus cells. In addition, some vein walls consisted of one or two layers of glomus cells. TCs, fibroblasts, muscle cells, and tunica media of the blood vessels, that present in the loose connective tissue surrounding the intertubular interstitium of camel epididymis, showed a positive reaction with vimentin. The endothelium of blood vessels and veins showed positive immunoreactivity for CD34 and vascular endothelial growth factor (VEGF). Furthermore, VEGF, CD34, and S100 proteins were expressed in dendritic cells (DCs) as well as TCs. The current data suggest the involvement of DCs and TCs in angiogenesis and a possible role for the interstitial components in creating an appropriate milieu for the full maturation of sperms.

中文翻译:

骆驼附睾微血管结构及其与特洛细胞和免疫细胞的关系

血管和血管周围细胞,包括端粒细胞 (TC) 和免疫细胞,在男性生育中发挥重要作用。目前的研究旨在详细描述除了单峰骆驼附睾的间质细胞成分外,还包含特殊调节装置的微血管结构。从 10 头临床健康的成熟骆驼 (Camelus dromedarius) 收集样品。使用光、免疫组织化学、免疫荧光和透射电子显微镜分析研究了 TC、附睾外周血管和免疫细胞的分布和特征。在附睾间质中显示出频繁的闭塞或节流小动脉,它们的中膜由球细胞组成。此外,一些静脉壁由一层或两层球状细胞组成。TCs、成纤维细胞、肌肉细胞和血管中膜,存在于骆驼附睾管间间质周围的疏松结缔组织中,与波形蛋白呈阳性反应。血管和静脉内皮对 CD34 和血管内皮生长因子 (VEGF) 呈阳性免疫反应。此外,VEGF、CD34 和 S100 蛋白在树突细胞 (DC) 和 TC 中表达。目前的数据表明 DCs 和 TCs 参与血管生成,以及间质成分在为精子完全成熟创造适当环境方面的可能作用。存在于骆驼附睾管间间质周围松散结缔组织中,与波形蛋白呈阳性反应。血管和静脉内皮对 CD34 和血管内皮生长因子 (VEGF) 呈阳性免疫反应。此外,VEGF、CD34 和 S100 蛋白在树突细胞 (DC) 和 TC 中表达。目前的数据表明 DCs 和 TCs 参与血管生成,以及间质成分在为精子完全成熟创造适当环境方面的可能作用。存在于骆驼附睾管间间质周围松散结缔组织中,与波形蛋白呈阳性反应。血管和静脉内皮对 CD34 和血管内皮生长因子 (VEGF) 呈阳性免疫反应。此外,VEGF、CD34 和 S100 蛋白在树突细胞 (DC) 和 TC 中表达。目前的数据表明 DCs 和 TCs 参与血管生成,以及间质成分在为精子完全成熟创造适当环境方面的可能作用。S100 和 S100 蛋白在树突细胞 (DC) 和 TC 中表达。目前的数据表明 DCs 和 TCs 参与血管生成,以及间质成分在为精子完全成熟创造适当环境方面的可能作用。S100 和 S100 蛋白在树突细胞 (DC) 和 TC 中表达。目前的数据表明 DCs 和 TCs 参与血管生成,以及间质成分在为精子完全成熟创造适当环境方面的可能作用。
更新日期:2020-07-15
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