Ageing is characterized by a progressive loss of physiological integrity, leading to impaired function and increased vulnerability to death1. Despite rapid advances over recent years, many of the molecular and cellular processes that underlie the progressive loss of healthy physiology are poorly understood2. To gain a better insight into these processes, here we generate a single-cell transcriptomic atlas across the lifespan of Mus musculus that includes data from 23 tissues and organs. We found cell-specific changes occurring across multiple cell types and organs, as well as age-related changes in the cellular composition of different organs. Using single-cell transcriptomic data, we assessed cell-type-specific manifestations of different hallmarks of ageing—such as senescence3, genomic instability4 and changes in the immune system2. This transcriptomic atlas—which we denote Tabula Muris Senis, or ‘Mouse Ageing Cell Atlas’—provides molecular information about how the most important hallmarks of ageing are reflected in a broad range of tissues and cell types. A single-cell transcriptomic atlas across the lifespan of the mouse, denoted Tabula Muris Senis, provides molecular information about the hallmarks of ageing in a range of tissues and cell types.

中文翻译：

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单细胞转录组图谱描绘了小鼠衰老组织的特征

衰老的特点是生理完整性逐渐丧失，导致功能受损并增加死亡风险1。尽管近年来取得了快速进展，但对导致健康生理机能逐渐丧失的许多分子和细胞过程仍知之甚少2。为了更好地了解这些过程，我们在此生成了小家鼠整个生命周期的单细胞转录组图谱，其中包括来自 23 个组织和器官的数据。我们发现多种细胞类型和器官发生细胞特异性变化，以及不同器官细胞组成的与年龄相关的变化。利用单细胞转录组数据，我们评估了不同衰老标志的细胞类型特异性表现，例如衰老3、基因组不稳定4和免疫系统变化2。这个转录组图谱（我们称之为 Tabula Muris Senis，或“小鼠衰老细胞图谱”）提供了有关衰老最重要标志如何在广泛的组织和细胞类型中反映的分子信息。小鼠整个生命周期的单细胞转录组图谱（称为 Tabula Muris Senis）提供了有关一系列组织和细胞类型衰老特征的分子信息。