A core problem in wound healing – with both fundamental and technological significance – concerns the rational design of bioactive and moist microenvironments. Here, we design a new class of zwitterionic betaine-incorporated collagen sponges (BET@COL) with integrated anti-oxidation and anti-inflammatory properties for promoting wound healing in a full-thickness wound model. The presence of zwitterionic betaine in a 3D network structure of collagen enables tightly bound and locked water molecules inside sponges via ionic solvation and confinement effect, while the integration of this amino acid also empowers the sponge with anti-oxidation and anti-inflammatory functions. In vitro results demonstrated that BET@COL collagen sponges strongly preserved water content up to 33.78 ± 0.78% at the 80th min at 37°C (only 0.44 ± 0.18% in control), and also exhibited high cell biocompatibility. Further, BET@COL collagen sponges with different betaine contents were applied to a full-thickness cutaneous wound model in mice, followed by a systematical evaluation and comparison of the effect of preserved water on wound healing efficiency in vivo. The optimal BET@COL collagen sponges were able to maintain high water content (e.g., moist microenvironment), suppress oxidative stress, improve anti-inflammation, all of which impose synergetic healing effects to promote wound closure, granulation formation, re-epithelization, collagen deposition and angiogenesis. This work demonstrates a new material as a promising candidate for wound dressing.

具有基本和技术意义的伤口愈合的核心问题涉及生物活性和潮湿微环境的合理设计。在这里，我们设计了一种新型的两性离子结合甜菜碱的胶原蛋白海绵（BET @ COL），具有集成的抗氧化和抗炎特性，可促进全层伤口模型中的伤口愈合。两性离子甜菜碱在胶原蛋白的3D网络结构中的存在通过离子溶剂化和封闭作用使海绵内部的水分子紧密结合和锁定，而该氨基酸的整合还使海绵具有抗氧化和抗发炎的功能。体外结果表明，BET @ COL胶原海绵在37°C下第80分钟时可将水分保持至33.78±0.78％（对照组仅为0.44±0.18％），并且还具有较高的细胞生物相容性。此外，将具有不同甜菜碱含量的BET @ COL胶原海绵应用于小鼠全层皮肤创面模型，然后系统评价和比较保水剂对创面愈合效率的影响。体内。最佳的BET @ COL胶原蛋白海绵能够保持高水分含量（例如，潮湿的微环境），抑制氧化应激，改善抗炎作用，所有这些都具有协同愈合作用，从而促进伤口闭合，肉芽形成，再上皮化，胶原蛋白沉积和血管生成。这项工作证明了一种新材料有望成为伤口敷料的候选材料。