Candida albicans is the most common human fungal pathogen, and with the increase in resistance rates worldwide, it is necessary to search for new pharmacological alternatives. Lavandula dentata L. essential oil is recognized as having antimicrobial properties. However, its effect against fungal biofilms has been poorly described. C. albicans-related infections involve the development of biofilms, which are highly resistant to conventional antifungals. In this work, we evaluated the antibiofilm effect of L. dentata L. essential oil against C. albicans. First, we characterized the essential oil by gas chromatography-mass spectrometry. The antifungal effect on C. albicans reference strains was evaluated by a disk diffusion assay and the minimal inhibitory concentration was obtained through a microdilution assay. The effect of the essential oil on the adhesion ability of C. albicans was determined through a crystal violet assay, and morphogenesis inhibition was assessed by light microscopy. The effect of the essential oil on the microarchitecture of biofilms was evaluated through scanning electron microscopy. Finally, the antibiofilm effect was evaluated through an adapted biofilm scratch assay and XTT viability assay. The main constituent of the essential oil was the monoterpenoid eucalyptol (60%). The essential oil presented minimal inhibitory concentrations of 156 and 130 µg/mL against two strains assayed. This minimal inhibitory concentration inhibited adhesion, morphogenesis, biofilm formation, altered microarchitecture, and decreased the viability of established biofilms formed on abiotic surfaces for both strains assayed. This study demonstrates that the essential oil from L. dentata could be a promising treatment against C. albicans biofilms.

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智利薰衣草精油的化学性质及其对白色念珠菌的抗生物膜作用

白色念珠菌是最常见的人类真菌病原体，随着全球耐药率的增加，有必要寻找新的药理学替代品。Lavandula dentata L. 精油被认为具有抗菌特性。然而，它对真菌生物膜的作用却鲜为人知。白色念珠菌相关感染涉及生物膜的形成，生物膜对常规抗真菌药具有高度抗性。在这项工作中，我们评估了 L. dentata L. 精油对白色念珠菌的抗生物膜作用。首先，我们通过气相色谱-质谱法表征了精油。对白色念珠菌参考菌株的抗真菌作用通过圆盘扩散试验评估，最小抑制浓度通过微量稀释试验获得。通过结晶紫测定确定精油对白色念珠菌粘附能力的影响，并通过光学显微镜评估形态发生抑制。通过扫描电子显微镜评估精油对生物膜微结构的影响。最后，通过适应的生物膜划痕试验和 XTT 活力试验评估了抗生物膜效果。精油的主要成分是单萜桉树脑（60%）。精油对所检测的两种菌株的最低抑菌浓度分别为 156 和 130 µg/mL。这种最小抑制浓度抑制了粘附、形态发生、生物膜形成、微结构改变，并降低了在非生物表面上形成的已测定生物膜的活力。