The Golgi is surrounded by a ribosome‐excluding matrix. Recently, we reported that the cis‐Golgi‐localized golgin GM130 can phase‐separate to form dynamic, liquid‐like condensates in vitro and in vivo. Here, we show that the overexpression of each of the remaining cis (golgin160, GMAP210)‐ and trans (golgin97, golgin245, GCC88, GCC185)‐golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB‐SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed condensates. These findings support the hypothesis that, in addition to their established roles as vesicle tethers, phase separation may be a common feature of the golgin family that contributes to Golgi organization.

中文翻译：

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golgin 家族表现出在活细胞中形成凝聚物的倾向

高尔基体被排除核糖体的基质包围。最近，我们报道了顺式高尔基体定位的高尔金 GM130 可以在体外和体内相分离形成动态的液体状凝聚物。在这里，我们展示了剩余的每一个顺式 (golgin160, GMAP210)-和反式 (golgin97, golgin245, GCC88, GCC185)-golgins 的过表达导致新的蛋白质缩合物。GM130 冷凝物的聚焦离子束扫描电子显微镜 (FIB-SEM) 图像揭示了具有分支水通道的复杂内部组织。在同一细胞中过表达的高尔金对形成不同的并列凝聚物。这些发现支持这样的假设，即除了它们作为囊泡系链的既定作用外，相分离可能是高尔基家族的共同特征，有助于高尔基组织。