Online databases containing genetic information are crucial to extract new candidate genes from existing data and web‐based resources. The objective of this study was, therefore, to predict putative candidate genes associated with resistance to SBR in line UG‐5 and understand their functions using different bioinformatics tools from the online available databases. The physical positions for the flanking markers of the identified putative QTLs were searched on the SoyBase database genome browser based on Glyma 1.01 assembly. The putative candidate genes and annotated functions of the surrounding genes were discovered in the vicinity using SoyBase and Phytozome databases. A total of 18 putative candidate genes were predicted on approximately 482.7 kb region of QTL‐3 (chromosome 18), among which, six putative candidate genes were found to encode leucine‐rich repeat (LRR), Ser/Thr protein phosphatase, leucine‐rich repeat receptor‐like protein kinase (LRR‐RLK) and chitinase‐related proteins, which are associated with plant defence signalling pathways. Moreover, F‐box and leucine‐rich repeat, glycosyltransferase family member and serine/threonine‐protein phosphatase 2A catalytic subunit coding genes were predicted on the novel putative QTL detected on chromosome 9. This information could, therefore, be used for further prediction and annotation of candidate genes from sequenced regions of line UG‐5 as these putative candidate genes were predicted from the Glyma 1.01 assembly.

中文翻译：

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UG-5品系与大豆锈病（Phakopsora pachyrhizi）抗性相关的候选基因的预测

包含遗传信息的在线数据库对于从现有数据和基于Web的资源中提取新的候选基因至关重要。因此，这项研究的目的是预测UG-5品系中与SBR抗性相关的推定候选基因，并使用在线数据库中的不同生物信息学工具了解其功能。在基于Glyma 1.01程序集的SoyBase数据库基因组浏览器中搜索已确定推定QTL侧翼标记的物理位置。使用SoyBase和Phytozome数据库在附近发现了假定的候选基因和周围基因的注释功能。在QTL-3的大约482.7 kb区域（第18号染色体）上预测了总共18个推定的候选基因，其中，发现六个推定的候选基因编码富含亮氨酸的重复序列（LRR），Ser / Thr蛋白磷酸酶，富含亮氨酸的重复受体样蛋白激酶（LRR‐RLK）和几丁质酶相关蛋白，它们与植物防御信号通路相关。此外，在第9号染色体上检测到的新型假定QTL上预测了F-box和富含亮氨酸的重复序列，糖基转移酶家族成员和丝氨酸/苏氨酸蛋白磷酸酶2A催化亚基编码基因。因此，该信息可用于进一步的预测和注释来自UG-5行测序区域的候选基因，因为这些推定的候选基因是从Glyma 1.01装配中预测的。与植物防御信号通路相关。此外，在第9号染色体上检测到的新型推定QTL上预测了F-box和富含亮氨酸的重复序列，糖基转移酶家族成员和丝氨酸/苏氨酸蛋白磷酸酶2A催化亚基编码基因。因此，该信息可用于进一步的预测和注释来自UG-5行测序区域的候选基因，因为这些推定的候选基因是从Glyma 1.01装配中预测的。与植物防御信号通路相关。此外，在第9号染色体上检测到的新型假定QTL上预测了F-box和富含亮氨酸的重复序列，糖基转移酶家族成员和丝氨酸/苏氨酸蛋白磷酸酶2A催化亚基编码基因。因此，该信息可用于进一步的预测和注释来自UG-5行测序区域的候选基因，因为这些推定的候选基因是从Glyma 1.01装配中预测的。