Systematic studies on the influence of selected ring–oxidized (7α–hydroxycholesterol, 7α−OH; 7β–hydroxycholesterol, 7β−OH; 7–ketocholesterol, 7–K) and chain–oxidized (25−OH) sterols on lipid layer of myelin were performed. Myelin sheath was modeled as five–component Langmuir monolayer (Chol:PE:SM:PS:PC 50:20:12:9:9). Particular oxysterols have been incorporated into the model myelin sheath by replacing cholesterol totally or partially (1:1). The effect of oxysterol incorporation was characterized with surface pressure and electric surface potential – area isotherms and visualized with Brewster angle microscopy (BAM) and atomic force microscopy (AFM). It has been noticed that model myelin loses its homogeneous structure (due to the appearance of domains) at physiological bilayer conditions (30–35 mN/m). In the presence of oxysterols, the fluidity of myelin model increases and the organization of lipids is altered, which is reflected in the decrease of electric surface potential changes (ΔV). The strongest myelin/oxysterol interactions have been observed for 7–K and 25−OH, being the most cytotoxic oxysterols found in biological tests.

对选定的环氧化（7α-羟基胆固醇，7α-OH;7β-羟基胆固醇，7β-OH; 7-酮胆固醇，7-K）和链氧化（25-OH）甾醇对髓磷脂脂质层影响的系统研究被执行。髓鞘被建模为五组分Langmuir单层（Chol：PE：SM：PS：PC 50：20：12：9：9）。通过全部或部分替换胆固醇（1：1），特定的甾醇已被掺入模型髓鞘中。用表面压力和表面电位-面积等温线表征了氧固醇的掺入效果，并用Brewster角显微镜（BAM）和原子力显微镜（AFM）进行了可视化。已经注意到，模型髓磷脂在生理双层条件（30-35 mN / m）下会失去其同质结构（由于域的出现）。在氧固醇存在下，髓磷脂模型的流动性增加，脂质的组织发生改变，这反映在表面电势变化（ΔV）的减小上。对于7–K和25–OH，已观察到最强的髓磷脂/氧固醇相互作用，是生物学测试中发现的最具细胞毒性的氧固醇。