In the separation of strongly polar antioxidant compounds from natural products using high-speed counter-current chromatography that is target-guided by 2,2-diphenyl-1-picrylhydrazyl high-performance liquid chromatography experimentation, low adsorption ability is encountered due to the strong polarity of the target compounds. In this study, a strategy of novel partition coefficient value calculation was proposed for overcoming this problem. The partition coefficient value was expressed as the ratio of the antioxidant activities of the upper phase and the lower phase. This strategy was used in high-speed counter-current chromatography with a hydrophilic organic/salt-containing aqueous two-phase system for bioassay-guided separation of strongly polar antioxidant compounds from Lycium barbarum L. The antioxidant activity was determined via the radical scavenging activity method using 2,2-diphenyl-1-picrylhydrazyl radicals. A hydrophilic organic/salt-containing aqueous two-phase system of 95% EtOH - sat. (NH₄)₂SO₄ (1:1.8, v/v) was successfully used to separate Lycium barbarum L. extract. Five fractions were collected via high-speed counter-current chromatography separation. The antioxidant activity of the third fraction was the highest. Three compounds were separated via MCI gel column chromatography and Sephadex LH-20 column chromatography from the third fraction, and their antioxidant activities were determined. The antioxidant activities of the three compounds were higher than that of the third fraction. These results demonstrate that this strategy can be used to separate strongly polar antioxidant compounds from natural products.

在以2,2-二苯基-1-吡啶并肼基高效液相色谱为目标的高速逆流色谱法中从天然产物中分离强极性抗氧化剂化合物时，由于吸附力强，遇到的吸附能力低目标化合物的极性。在这项研究中，提出了一种新颖的分配系数值计算策略来克服这一问题。分配系数值表示为上层相和下层相的抗氧化活性的比率。该策略用于带有亲水性有机/含盐的水相两相系统的高速逆流色谱法中，用于从枸杞中进行生物测定指导的强极性抗氧化剂化合物的分离L.使用2，2-二苯基-1-吡啶并肼基通过自由基清除活性方法测定抗氧化剂活性。亲水性有机/含盐的含水两相系统，含95％乙醇-饱和。（NH ₄）₂ SO ₄（1：1.8，v / v）已成功用于分离枸杞L.提取物。通过高速逆流色谱分离收集五个级分。第三部分的抗氧化活性最高。通过MCI凝胶柱色谱法和Sephadex LH-20柱色谱法从第三部分中分离出三种化合物，并确定了它们的抗氧化活性。三种化合物的抗氧化活性均高于第三部分。这些结果表明，该策略可用于从天然产物中分离强极性抗氧化剂化合物。