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A transcription factor glial cell missing (Gcm) in the freshwater crayfish Pacifastacus leniusculus.
Developmental & Comparative Immunology ( IF 2.9 ) Pub Date : 2020-07-15 , DOI: 10.1016/j.dci.2020.103782
Kingkamon Junkunlo 1 , Kenneth Söderhäll 2 , Irene Söderhäll 2
Affiliation  

The transcription factor glial cell missing, Gcm, is known to be an important protein in the determination of glial cell fate as well as embryonic plasmatocyte differentiation in Drosophila melanogaster. So far, no function for Gcm in crustaceans has been reported. In this study, we show the cDNA sequence of a Gcm homologue in the freshwater crayfish Pacifastacus leniusculus. The P. leniusculus Gcm transcript is expressed exclusively in brain and nervous tissue, and by in situ hybridization we show that the expression is restricted to a small number of large cells with morphology similar to neurosecretory cells. Furthermore, we show that the expression of Gcm coincides with the expression of a Repo homologue, that is induced in expression by Gcm in Drosophila. Moreover, the Gcm transcript is increased shortly and transiently after injection of cystamine, a substance that inhibits transglutaminase and also strongly affects the movement behavior of crayfish. This finding of Gcm transcripts in a subpopulation of brain cells in very low numbers may enable more detailed studies about Gcm in adult crustaceans.



中文翻译:

淡水小龙虾 Pacifastacus leniusculus 中的转录因子神经胶质细胞缺失 (Gcm)。

已知转录因子胶质细胞缺失 Gcm 是确定黑腹果蝇中胶质细胞命运以及胚胎浆细胞分化的重要蛋白质。到目前为止,还没有关于甲壳类动物 Gcm 功能的报道。在这项研究中,我们展示了淡水小龙虾Pacifastacus leniusculus 中 Gcm同源物的 cDNA 序列。该P. leniusculus GCM成绩单在大脑和神经组织特异表达,并通过原位杂交我们表明表达仅限于少数具有类似于神经分泌细胞的形态的大细胞。此外,我们表明 Gcm 的表达与 Repo 同源物的表达一致,即在果蝇中由 Gcm 诱导表达。此外,注射胱胺(一种抑制转谷氨酰胺酶并强烈影响小龙虾运动行为的物质)后,Gcm 转录物会在短时间内和短暂地增加。在数量非常少的脑细胞亚群中 Gcm 转录本的这一发现可能有助于对成年甲壳类动物中的 Gcm 进行更详细的研究。

更新日期:2020-07-24
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