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DNA dosimeter measurements of beam profile using a novel simultaneous processing technique.
Applied Radiation and Isotopes ( IF 1.6 ) Pub Date : 2020-07-15 , DOI: 10.1016/j.apradiso.2020.109316
B Bui 1 , K McConnell 1 , M Obeidat 1 , D Saenz 1 , N Papanikolaou 1 , E Y Shim 1 , N Kirby 1
Affiliation  

A DNA dosimeter (DNAd) was previously developed that uses double-strand breaks (DSB) to measure dose. This dosimeter has been tested to measure dose in scenarios where transient-charged particle equilibrium (TCPE) has been established. The probability of double strand break (PDSBo), which is the ratio of broken double-stranded DNA (dsDNA) to the initial unbroken dsDNA in the dosimeter, was used to quantify DSBs and related to dose. The goal of this work is to produce a new technique to process and analyze the DNAd and quantify DNA-DSBs. This technique included simultaneously processing multiple DNAds and also establishing a new form to the probability of double strand break (PDSBn), which was then used to test the DNAd in a non-TCPE condition by taking beam penumbra measurements. The technique utilized a 384-well plate, and the measurements were made at the edge of a 10 × 10 cm field and compared to film measurements. During these penumbra measurements, while observing the positional differences in the higher gradient region at 4.1 and 4.55 cm from the center of the radiation field, the distance to agreement of PDSBo to film were 0.38 cm and 0.26 cm while the distance to agreement of PDSBn to film were 0.11 cm and 0.06 cm, respectively. Finally, the developed new separation technique reduced the time needed for the analysis of 25 samples from 200 min to 30 min.



中文翻译:

使用新型同步处理技术对光束轮廓进行 DNA 剂量计测量。

之前开发的 DNA 剂量计 (DNAd) 使用双链断裂 (DSB) 来测量剂量。该剂量计已经过测试,可在已建立瞬态带电粒子平衡 (TCPE) 的情况下测量剂量。双链断裂概率 (PDSB o ),即剂量计中断裂双链 DNA (dsDNA) 与初始未断裂 dsDNA 的比率,用于量化 DSB 并与剂量相关。这项工作的目标是产生一种新技术来处理和分析 DNAd 并量化 DNA-DSB。该技术包括同时处理多个 DNAd 并建立一种新形式的双链断裂概率 (PDSB n),然后通过进行光束半影测量来测试非 TCPE 条件下的 DNAd。该技术使用 384 孔板,在 10 × 10 cm 视野的边缘进行测量,并与胶片测量进行比较。在这些半影测量期间,在观察距辐射场中心 4.1 和 4.55 厘米的较高梯度区域的位置差异时,PDSB o与胶片的一致距离为 0.38 厘米和 0.26 厘米,而 PDSB 的一致距离为ñ到膜分别0.11厘米和0.06厘米,。最后,开发的新分离技术将分析 25 个样品所需的时间从 200 分钟减少到 30 分钟。

更新日期:2020-07-15
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