Abstract—The content of crude protein in leaves and seeds of vegetable soybean, an important legume crop used in a functional and healthy nutrition, has been evaluated by a spectrophotometric analysis. Breeding forms of soybean were grown on an experimental field and in a greenhouse of the Federal Scientific Vegetable Center (Moscow oblast). In this 3-year work, the crude protein accumulation in seeds of vegetable soybean at the R8-stage stage under conditions of the Central region of the Non-Chernozem Soil Zone of Russia (55th parallel north) were first studied. No significant difference between the vegetable and oil-seed soybean forms was observed in relation to the protein content in leaves at the R6-stage phase; the average value for vegetable samples was 8.1%. At this phase, the level of protein accumulation in seeds of all samples was almost equal (27.5–28.4%). According to the data of 3-year observations, vegetable soybean accumulated the higher total protein (+15.7% in relative values) at the R8-stage phase, which may be explained by hereditary factors. The maximum crude protein content in seeds was observed in breeding lines Obrazets A, Hidaka, and Cha Kura Kake; the average value for these lines reached 47.4% in absolute units. Under studied conditions, a significant accumulation of crude protein was observed in leaves and seeds of vegetable soybean during both phases of generative development, which may provide great prospects for the use of these samples in the production of functional food products with a high protein content.

中文翻译：

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非白藜芦土壤带中部种植的蔬菜大豆样品中的粗蛋白质积累

摘要-已经通过分光光度分析法评估了蔬菜大豆的叶子和种子中粗蛋白的含量，蔬菜豆是功能和健康营养中一种重要的豆类作物。大豆的育种形式在联邦科学蔬菜中心（莫斯科州）的实验田和温室中生长。在这项为期三年的工作中，首先研究了在俄罗斯非切尔诺泽姆土壤带中部地区（平行第55个平行北部）的条件下，R8期植物菜豆种子中的粗蛋白积累。在R6阶段，相对于叶片中的蛋白质含量，未发现植物形式和油料大豆形式之间的显着差异。蔬菜样品的平均值为8.1％。在这个阶段 所有样品种子中蛋白质的积累水平几乎相等（27.5–28.4％）。根据3年观察的数据，蔬菜大豆在R8期阶段积累了较高的总蛋白质（相对值+ 15.7％），这可能是由遗传因素引起的。在育种系Obrazets A，Hidaka和Cha Kura Kake中观察到了种子中最大的粗蛋白含量。这些线的平均值以绝对单位计达到47.4％。在研究的条件下，在生成发育的两个阶段中，蔬菜大豆的叶子和种子中均观察到粗蛋白的大量积累，这可能为将这些样品用于生产高蛋白含量的功能性食品提供了广阔的前景。蔬菜大豆在R8期阶段积累了较高的总蛋白质（相对值+ 15.7％），这可由遗传因素解释。在育种系Obrazets A，Hidaka和Cha Kura Kake中观察到了种子中最大的粗蛋白含量。这些线的平均值以绝对单位计达到47.4％。在研究的条件下，在生成发育的两个阶段中，蔬菜大豆的叶子和种子中均观察到粗蛋白的大量积累，这可能为将这些样品用于生产高蛋白含量的功能性食品提供了广阔的前景。蔬菜大豆在R8期阶段积累了较高的总蛋白质（相对值+ 15.7％），这可由遗传因素解释。在育种系Obrazets A，Hidaka和Cha Kura Kake中观察到种子中最大的粗蛋白含量。这些线的平均值以绝对单位计达到47.4％。在研究的条件下，在生成发育的两个阶段中，蔬菜大豆的叶子和种子中均观察到粗蛋白的大量积累，这为将这些样品用于生产高蛋白含量的功能性食品提供了广阔的前景。还有查库拉。这些线的平均值以绝对单位计达到47.4％。在研究的条件下，在生成发育的两个阶段中，蔬菜大豆的叶子和种子中均观察到粗蛋白的大量积累，这可能为将这些样品用于生产高蛋白含量的功能性食品提供了广阔的前景。还有查库拉。这些线的平均值以绝对单位计达到47.4％。在研究的条件下，在生成发育的两个阶段中，蔬菜大豆的叶子和种子中均观察到粗蛋白的大量积累，这可能为将这些样品用于生产高蛋白含量的功能性食品提供了广阔的前景。