Abstract

The kinetics of meiotic chromatin modifications and the function of lipidome in the oocytes that have completed their in vivo or in vitro growth phase after a prolonged culture period have been analyzed in order to improve the efficiency of a technology for extracorporeal maturation of porcine donor-derived oocytes. It is revealed that a prolonged period (up to 50 h) of culturing in the Sage Cleavage medium (CooperSurgical, United States) with the addition of 5% Serum Protein Substitute supplement (Cooper Surgical, United States) and 10 IU human chorionic gonadotropin can provoke destruction of chromatin of BCB⁺-positive and BCB^–-negative oocytes (76 vs. 50%, P < 0.01). The age-associated porcine oocyte processes are usually followed by changes in lipid droplet morphology, since the granules tend to transform into clusters. A high percent of BCB^–-negative oocytes containing normal chromatin in metaphase 2 can indicate the capability of oocytes that have not completed their in vivo growth phases to complete it in vitro with the subsequent meiotic maturation. The produced data may be used to model the maturation systems for porcine female gametes that have not completed their in vivo growth phases.

中文翻译：

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延长培养时间后已完成其体内或体外生长期的猪卵母细胞的减数分裂成熟和脂质组的功能

摘要

为了提高猪供体来源的体外成熟技术的效率，分析了延长培养时间后已完成体内或体外生长期的卵母细胞的减数分裂染色质修饰动力学和脂质组的功能。卵母细胞。可以发现，在鼠尾草裂解培养基（美国CooperSurgical）中添加5％的血清蛋白替代品（美国Cooper Surgical）和10 IU人绒毛膜促性腺激素可以延长培养时间（长达50小时）。引起对BCB ⁺阳性和BCB ^–阴性卵母细胞染色质的破坏（76 vs. 50％，P<0.01）。与年龄相关的猪卵母细胞过程通常伴随着脂质液滴形态的变化，因为颗粒趋于转变成簇。BCB的高百分比^-含在中期2正常染色质阴性的卵母细胞可以指示还没有完成其在体内的生长阶段与随后的减数分裂成熟完成它在体外卵母细胞的能力。产生的数据可用于为尚未完成其体内生长阶段的猪雌配子的成熟系统建模。