Aluminum cookware are widely used in many parts of the world. Data is increasing on the leaching of toxic metals from aluminum cookware into food and drink. In the present study, cytogenotoxicity of water boiled in three different aluminum pots (new, 3-year-old, and 6-year-old) in onion root tip’s dividing cells was evaluated using the Allium cepa assay. The concentrations of Pb, As, Cd, Cu, Ni, and Al in the samples were also analyzed. Onion bulbs were grown in the boiled water samples, while tap water served as the control. Cytological and genetic analyses were carried out after 48 h, while analysis of inhibition of root length was carried out after 72 h. The results showed a significant (p < 0.05) cell proliferation and root growth inhibition compared with the control, which is dependent on the duration of use of the aluminum pots. The boiled water samples also caused modification of the root morphology as well as chromosomal aberrations which include sticky chromosomes, anaphase bridge, and disturbed spindle. The highest cytogenotoxicity was observed in the 6-year-old aluminum pot and the least in the new aluminum pot. Pb, As, Cd, Cu, Ni, and Al analyzed in the samples, with the highest concentrations in the 6-year-old aluminum pot, were believed to be responsible for the cytogenotoxicity observed in the A. cepa assay. The data of this study are indications that the aluminum pot–boiled water contains substances with the potential to be cytotoxic and cause mutations in somatic cells of A. cepa.

中文翻译：

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铝制炊具使用时间对其金属浸出性和细胞遗传毒性的影响在洋葱实验中

铝制炊具在世界许多地方被广泛使用。关于有毒金属从铝制炊具浸出到食品和饮料中的数据正在增加。在本研究中，在三个不同的铝锅（新的、3 年的和 6 年的）中煮沸的水在洋葱根尖分裂细胞中的细胞遗传毒性使用洋葱分析法进行了评估。还分析了样品中 Pb、As、Cd、Cu、Ni 和 Al 的浓度。洋葱球茎在开水样品中生长，而自来水作为对照。48 小时后进行细胞学和遗传分析，72 小时后进行根长抑制分析。结果表明，与对照相比，细胞增殖和根生长受到显着的（p < 0.05）抑制，这取决于铝盆的使用时间。沸水样品还导致根形态的改变以及染色体畸变，包括粘性染色体、后期桥和扰动的纺锤体。在 6 年的铝锅中观察到最高的细胞遗传毒性，在新的铝锅中观察到的最少。样品中分析的 Pb、As、Cd、Cu、Ni 和 Al（在 6 年前的铝罐中浓度最高）被认为是导致在 A. cepa 测定中观察到的细胞遗传毒性的原因。这项研究的数据表明，铝锅煮沸的水含有可能具有细胞毒性并导致 A. cepa 体细胞突变的物质。在 6 年的铝锅中观察到最高的细胞遗传毒性，在新的铝锅中观察到的最少。样品中分析的 Pb、As、Cd、Cu、Ni 和 Al（在 6 年前的铝罐中浓度最高）被认为是导致在 A. cepa 测定中观察到的细胞遗传毒性的原因。这项研究的数据表明，铝锅煮沸的水含有可能具有细胞毒性并导致 A. cepa 体细胞突变的物质。在 6 年的铝锅中观察到最高的细胞遗传毒性，在新的铝锅中观察到的最少。样品中分析的 Pb、As、Cd、Cu、Ni 和 Al（在 6 年前的铝罐中浓度最高）被认为是导致在 A. cepa 测定中观察到的细胞遗传毒性的原因。这项研究的数据表明，铝锅煮沸的水含有可能具有细胞毒性并导致 A. cepa 体细胞突变的物质。