This study reports ascorbic acid adsorption/release performance and antibacterial activity of chitosan composite microcapsules containing terpolymer of glycidyl methacrylate (GMA), maleic anhydride (MA) and N-tert-butylacrylamide (NTBA). The effects of amount of chitosan composite microcapsules, solution pH, ascorbic acid concentration and contact time on the adsorption of ascorbic acid by chitosan-ter (GMA-MA-NTBA) polymer microcapsules were studied. The maximum ascorbic acid adsorption was observed for 0.075 g of microcapsules under following conditions; ascorbic acid concentration: 10 mg L⁻¹, pH 7.35, temperature: 35 °C and contact time: 1 h. Antibacterial activity of chitosan-ter (GMA-MA-NTBA) polymer microcapsules was tested against Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25113, Klebsiella pneumoniae ATCC 700603, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Salmonella enteritidis ATCC 13076 bacterial strains. In vitro antibacterial studies demonstrated that chitosan microcapsules with 0.25% ter (GMA-MA-NTBA) polymer (by mass) were more effective on inhibiting of bacterial growth. Plain glutaraldehyde-chitosan beads showed no antibacterial activity, indicating that ter(GMA-MA-NTBA) polymer content was responsible for the antibacterial activity of the chitosan-ter(GMA-MA-NTBA) polymer microcapsules.

Graphic Abstract

中文翻译：

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壳聚糖三（GMA-MA-NTBA）聚合物微胶囊的抗坏血酸吸附释放性能和抑菌活性

该研究报告抗坏血酸吸附/释放性能和含有甲基丙烯酸缩水甘油酯（GMA），马来酸酐（MA）和三元共聚物壳聚糖复合微胶囊的抗菌活性ñ -叔-butylacrylamide（NTBA）。研究了壳聚糖复合微胶囊的用量，溶液的pH值，抗坏血酸的浓度和接触时间对壳聚糖三聚体（GMA-MA-NTBA）聚合物微胶囊吸附抗坏血酸的影响。在以下条件下，对于0.075 g微胶囊，观察到最大的抗坏血酸吸附；抗坏血酸浓度：10 mg L ^-1，pH 7.35，温度：35°C，接触时间：1 h。测试了壳聚糖三萜（GMA-MA-NTBA）聚合物微胶囊的抗菌活性铜绿假单胞菌ATCC 27853，大肠杆菌ATCC 25113，肺炎克雷伯菌ATCC 700603，金黄色葡萄球菌ATCC 25923，粪肠球菌ATCC 29212和肠炎沙门氏菌ATCC 13076细菌菌株。体外抗菌研究表明，以质量百分比为0.25％的壳聚糖微胶囊（GMA-MA-NTBA）（质量）可更有效地抑制细菌生长。普通戊二醛-壳聚糖微珠未显示抗菌活性，表明叔（GMA-MA-NTBA）聚合物含量是壳聚糖-叔（GMA-MA-NTBA）聚合物微囊的抗菌活性。

图形摘要