Lung adenocarcinoma (LUAD) is the top prevalent histological kind of lung cancer worldwide. Recent evidences have demonstrated that Sauchinone plays an anticancer role in tumor cell invasion and migration. Therefore, we performed this investigation to explain the potential role of Sauchinone in LUAD as well as the potential mechanism involved. Cell counting kit 8 (CCK-8) and transwell experiments were implemented to measure the proliferative, invasive and migratory abilities of LUAD cells. qRT-PCR and Western blot were performed to detect the transfection efficiency of si-EIF4EBP1s. Additionally, Western blot was also implemented to evaluate the effect of Sauchinone on EIF4EBP1 expression level as well as cell cycle-related proteins. Our findings showed that Sauchinone remarkably suppressed the proliferative ability of LUAD cells in a dose-dependent and time-dependent manner. EIF4EBP1 was a candidate target gene of Sauchinone. EIF4EBP1 expression was increased in LUAD tissues, and its high expression induced a poorer prognosis of LUAD patients. EIF4EBP1 expression was positively associated with cell cycle in LUAD. Sauchinone treatment attenuated EIF4EBP1 expression and cell cycle-related protein levels. Knockdown of EIF4EBP1 repressed the proliferation, invasion and migration of LUAD cells; furthermore, Sauchinone stimulation enforced its inhibitory effect. Meanwhile, the treatment of Sauchinone intensified the arrest of cell cycle induced by EIF4EBP1 knockdown. To sum up, our discovery indicated that Sauchinone exerts an anticancer role through down-regulating EIF4EBP1 and mediating cell cycle in LUAD.

中文翻译：

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Sauchinone：一种预期的治疗剂介导的EIF4EBP1下调抑制了肺腺癌细胞的增殖，侵袭和迁移。

肺腺癌（LUAD）是全球范围内最常见的肺癌组织学类型。最近的证据表明，Sauchinone在肿瘤细胞的侵袭和迁移中起着抗癌作用。因此，我们进行了这项调查，以解释Sauchinone在LUAD中的潜在作用以及潜在的机制。进行细胞计数试剂盒8（CCK-8）和Transwell实验以测量LUAD细胞的增殖，侵袭和迁移能力。进行qRT-PCR和Western blot检测si-EIF4EBP1s的转染效率。此外，还进行了蛋白质印迹，以评估Sauchinone对EIF4EBP1表达水平以及细胞周期相关蛋白的影响。我们的发现表明，Sauchinone以剂量依赖性和时间依赖性方式显着抑制LUAD细胞的增殖能力。EIF4EBP1是Sauchinone的候选靶基因。EIF4EBP1在LUAD组织中表达增加，其高表达诱导LUAD患者的预后较差。EIF4EBP1表达与LUAD细胞周期呈正相关。Sauchinone处理可减弱EIF4EBP1表达和细胞周期相关蛋白水平。击倒EIF4EBP1抑制LUAD细胞的增殖，侵袭和迁移。此外，Sauchinone刺激增强了其抑制作用。同时，Sauchinone的治疗加剧了EIF4EBP1敲低诱导的细胞周期停滞。总结一下，