The presence of Burkholderia cepacia complex (BCC) strains has resulted in recalls of pharmaceutical products, since these opportunistic pathogens can cause serious infections. Rapid and sensitive diagnostic methods to detect BCC are crucial to determine contamination levels. We evaluated bacterial cultures, real-time PCR (qPCR), droplet digital PCR (ddPCR), and flow cytometry to detect BCC in nuclease-free water, in chlorhexidine gluconate (CHX) and benzalkonium chloride (BZK) solutions. Twenty BCC strains were each suspended (1, 10, 100, and 1000 CFU/ml) in autoclaved nuclease-free water, 10 μg/ml CHX, and 50 μg/ml BZK. Five replicates of each strain were tested at each concentration (20 strains × 4 concentrations × 5 replicates = 400 tests) to detect BCC using the aforementioned four methods. We demonstrated the potential of ddPCR and flow cytometry as more sensitive alternatives to culture-based methods to detect BCC in autoclaved nuclease-free water and antiseptics samples.

洋葱伯克霍尔德菌的存在复杂的（BCC）菌株已导致召回药品，因为这些机会病原体会引起严重的感染。快速，灵敏的诊断方法可检测BCC对确定污染水平至关重要。我们评估了细菌培养物，实时PCR（qPCR），液滴数字PCR（ddPCR）和流式细胞仪，以检测葡萄糖酸洗必泰（CHX）和苯扎氯铵（BZK）解决方案中无核酸酶的水中的BCC。将二十个BCC菌株分别悬浮（1、10、100和1000 CFU / ml）在高压灭菌的无核酸酶的水，10μg/ ml CHX和50μg/ ml BZK中。使用上述四种方法在每种浓度下测试每个菌株的五个重复（20个菌株×4个浓度×5个重复= 400个测试）以检测BCC。