Fluorescence assay and Raman spectroscopy were used to study the mechanisms of action of 2-isopropyl-5-methyl- 1,4-benzoquinone (thymoquinone) on HEp-2 human larynx carcinoma cells. Thymoquinone was found to have a more pronounced toxic effect than 1,4-benzoquinone and 2,3,5-trimethyl-1,4-benzoquinone. The action of thymoquinone leads to a decrease in the mitochondrial membrane potential and release of cytochrome c from the mitochondria, indicating activation of apoptosis of tumor cells through the mitochondrial-mediated pathway. These results indicate the possibility of using Raman spectroscopy in the study of programmed cell death.

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甲状腺素抗肿瘤作用的拉曼光谱研究

荧光分析法和拉曼光谱法用于研究2-异丙基-5-甲基-1，4-苯醌（胸腺醌）对人喉癌Hep-2细胞的作用机制。发现胸腺醌比1,4-苯醌和2,3,5-三甲基-1,4-苯醌具有更明显的毒性作用。胸腺醌的作用导致线粒体膜电位的降低和细胞色素c从线粒体的释放，表明肿瘤细胞通过线粒体介导的途径凋亡。这些结果表明在程序性细胞死亡研究中使用拉曼光谱的可能性。