The use of agro-industrial by-products as feedstock for selenium-enriched yeast biomass production is an innovative and low-cost method. Thus, the present study aims for the utilization of sugarcane bagasse and corn bran hydrolysates for the production of selenium-enriched yeast and the evaluation of the effect of selenium in growth and cell composition. The hydrolysates were obtained using acid pretreatment, and yeasts were evaluated in different medium compositions. All evaluated yeasts were able to grow in the presence of 15 mg/L selenium. S. cerevisiae strains presented the major tolerance to selenium and better relation between selenium uptake and cell growth, with maximum cell biomass production of 7.97 ± 0.24 g/L and maximum selenium uptake of 1193 ± 336 ppm and 99% cell viability. Selenium also enhanced the lipid and protein concentration in cell extracts obtained after mechanical disruption. Regarding agro-industrial hydrolysates, corn bran hydrolysate allowed a better growth, with the production of 4.25 ± 0.49 g/L of biomass enriched with 167 ± 18 ppm selenium and 100% of viability. Starchy and lignocellulosic biomasses presented potential as low-cost alternatives for single-cell protein contributing to the development of sustainable and economically viable technologies.

Graphical abstract

使用农业工业副产品作为富硒酵母生物量生产的原料是一种创新的低成本方法。因此，本研究的目的是利用甘蔗渣和玉米糠水解物生产富硒酵母，并评估硒对生长和细胞组成的影响。使用酸预处理获得水解产物，并在不同的培养基组成中评估酵母。所有评估的酵母都能够在15 mg / L硒的存在下生长。酿酒酵母菌株表现出对硒的主要耐受性以及硒摄取与细胞生长之间的更好关系，最大细胞生物量产量为7.97±0.24 g / L，最大硒摄取量为1193±336 ppm，细胞活力为99％。硒还增强了机械破坏后获得的细胞提取物中脂质和蛋白质的浓度。关于农用工业水解产物，玉米糠水解产物具有更好的生长性，产生4.25±0.49 g / L的生物质，富含167±18 ppm的硒和100％的活力。淀粉和木质纤维素生物质作为单细胞蛋白质的低成本替代品具有潜力，有助于发展可持续和经济上可行的技术。

图形概要