In this study, 5-amino-nicotinic acid derivatives (1–13) have been designed and synthesized to evaluate their inhibitory potential against α-amylase and α-glucosidase enzymes. The synthesized compounds (1–13) exhibited promising α-amylase and α-glucosidase activities. IC50 values for α-amylase activity ranged between 12.17 ± 0.14 to 37.33 ± 0.02 µg/mL ± SEM while for α-glucosidase activity the IC50 values were ranged between 12.01 ± 0.09 to 38.01 ± 0.12 µg/mL ± SEM. In particular, compounds 2 and 4–8 demonstrated significant inhibitory activities against α-amylase and α-glucosidase and the inhibitory potential of these compounds was comparable to the standard acarbose (10.98 ± 0.03 and 10.79 ± 0.17 µg/mL ± SEM, respectively). In addition, the impact of substituent on the inhibitory potential of these compounds was assessed to establish structure activity relationships. Studies in molecular simulations were conducted to better comprehend the binding properties of the compounds. All the synthesized compounds were extensively characterized with modern spectroscopic methods including 1H-NMR, 13C–NMR, FTIR, HR-MS and elemental analysis.

中文翻译：

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5-氨基烟酸衍生物的结构解析、分子对接、α-淀粉酶和α-葡萄糖苷酶抑制研究。

在本研究中，设计并合成了 5-氨基烟酸衍生物 (1-13)，以评估其对 α-淀粉酶和 α-葡萄糖苷酶的抑制潜力。合成的化合物 (1-13) 表现出有前景的 α-淀粉酶和 α-葡萄糖苷酶活性。α-淀粉酶活性的 IC50 值范围为 12.17 ± 0.14 至 37.33 ± 0.02 µg/mL ± SEM，而 α-葡萄糖苷酶活性的 IC50 值范围为 12.01 ± 0.09 至 38.01 ± 0.12 µg/mL ± SEM。特别是，化合物 2 和 4-8 对 α-淀粉酶和 α-葡萄糖苷酶表现出显着的抑制活性，并且这些化合物的抑制潜力与标准阿卡波糖相当（分别为 10.98 ± 0.03 和 10.79 ± 0.17 µg/mL ± SEM） 。此外，还评估了取代基对这些化合物的抑制潜力的影响，以建立结构活性关系。进行分子模拟研究是为了更好地理解化合物的结合特性。所有合成的化合物均采用现代光谱方法进行了广泛表征，包括 1H-NMR、13C-NMR、FTIR、HR-MS 和元素分析。