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ABI3 plays a role in de-novo root regeneration from Arabidopsis thaliana callus cells
Plant Signaling & Behavior ( IF 2.9 ) Pub Date : 2020-07-14 , DOI: 10.1080/15592324.2020.1794147 Sourabh Sengupta 1 , Ronita Nag Chaudhuri 1
Plant Signaling & Behavior ( IF 2.9 ) Pub Date : 2020-07-14 , DOI: 10.1080/15592324.2020.1794147 Sourabh Sengupta 1 , Ronita Nag Chaudhuri 1
Affiliation
ABSTRACT Developmental plasticity and the ability to regenerate organs during the life cycle are a signature feature of plant system. De novo organogenesis is a common mode of plant regeneration and may occur directly from the explant or indirectly via callus formation. It is now evident that callus formation occurs through the root development pathway. In fact, callus cells behave like a group of root primordium cells that are under the control of exogenous auxin. Presence or absence of auxin decides the subsequent fate of these cells. While in presence of external supplementation of auxin they are maintained as root primordia cells, absence of exogenous auxin induces the callus cells into patterning, differentiation and finally root emergence. Here we show that in absence of functional ABI3, a prominent member of the B3 superfamily of transcription factors, root regeneration is compromised in Arabidopsis callus cells. In culture medium free of any exogenous hormone supplementation, while adventitious root emergence and growth was prominently observed in wild type cells, no such features were observed in abi3-6 cells. Expression of auxin-responsive AUX1 and GH3 genes was significantly reduced in abi3-6 cells, indicating that auxin levels or distribution may be altered in absence of ABI3.
中文翻译:
ABI3 在拟南芥愈伤组织细胞从头再生中发挥作用
摘要 发育可塑性和生命周期中器官再生的能力是植物系统的标志性特征。从头器官发生是植物再生的常见模式,可以直接从外植体发生或通过愈伤组织形成间接发生。现在很明显,愈伤组织的形成是通过根发育途径发生的。事实上,愈伤组织细胞的行为就像一组受外源生长素控制的根原基细胞。生长素的存在与否决定了这些细胞随后的命运。虽然在外部补充生长素的情况下,它们作为根原基细胞维持,但没有外源生长素会诱导愈伤组织细胞形成图案、分化并最终出根。在这里我们表明,在没有功能性 ABI3 的情况下,作为 B3 转录因子超家族的重要成员,拟南芥愈伤组织细胞中的根再生受到损害。在不含任何外源激素补充剂的培养基中,虽然在野生型细胞中明显观察到不定根的出现和生长,但在 abi3-6 细胞中没有观察到这种特征。在 abi3-6 细胞中,生长素反应性 AUX1 和 GH3 基因的表达显着降低,表明在缺乏 ABI3 的情况下,生长素水平或分布可能会发生改变。
更新日期:2020-07-14
中文翻译:
ABI3 在拟南芥愈伤组织细胞从头再生中发挥作用
摘要 发育可塑性和生命周期中器官再生的能力是植物系统的标志性特征。从头器官发生是植物再生的常见模式,可以直接从外植体发生或通过愈伤组织形成间接发生。现在很明显,愈伤组织的形成是通过根发育途径发生的。事实上,愈伤组织细胞的行为就像一组受外源生长素控制的根原基细胞。生长素的存在与否决定了这些细胞随后的命运。虽然在外部补充生长素的情况下,它们作为根原基细胞维持,但没有外源生长素会诱导愈伤组织细胞形成图案、分化并最终出根。在这里我们表明,在没有功能性 ABI3 的情况下,作为 B3 转录因子超家族的重要成员,拟南芥愈伤组织细胞中的根再生受到损害。在不含任何外源激素补充剂的培养基中,虽然在野生型细胞中明显观察到不定根的出现和生长,但在 abi3-6 细胞中没有观察到这种特征。在 abi3-6 细胞中,生长素反应性 AUX1 和 GH3 基因的表达显着降低,表明在缺乏 ABI3 的情况下,生长素水平或分布可能会发生改变。