Abstract

With the advantages of mild reaction condition as well as high stereoselectivity and efficiency, chemoselective ligations including oxime chemistry, hydrazone chemistry, cycloaddition reaction, C-C multiple bond addition reaction, nucleophilic rings opening reaction, and alkynes-based reaction, have been applied in the field of biosensing. In this review, the roles of these chemoselective ligations for the construction of biosensors have been summarized. The ligations can serve for reactant preparation, interface modification, signal probe synthesis, molecular recognition, signal amplification, and output. Meanwhile, their recent applications for detection of various targets including small molecules, metal ions, protein, and nucleic acid so on, have been reviewed. Moreover, the development trend of these ligations in biosensing has also been prospected in this review.

摘要

肟化学、腙化学、环加成反应、CC多键加成反应、亲核开环反应、炔基反应等化学选择性连接反应具有反应条件温和、立体选择性和效率高等优点，已在该领域得到应用。的生物传感。在这篇综述中，总结了这些化学选择性连接在构建生物传感器中的作用。连接可用于反应物制备、界面修饰、信号探针合成、分子识别、信号放大和输出。同时，综述了它们近期在检测小分子、金属离子、蛋白质、核酸等多种靶标方面的应用。而且，