Absolute (molar) quantification of proteins determines their molar ratios in complexes, networks, and metabolic pathways. MS Western workflow is employed to determine molar abundances of proteins potentially critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric 264 kDa protein standard that covers, in total, 197 peptides from 43 proteins. The majority of proteins are independently quantified with two to four proteotypic peptides with the coefficient of variation of less than 15%, better than 1000‐fold dynamic range and sub‐femtomole sensitivity. Here, the molar abundance of proteins of the PT machinery and of the rhabdomere, the photosensitive organelle, is determined in eyes of wild‐type flies as well as in crumbs (crb) mutant eyes, which exhibit perturbed rhabdomere morphogenesis.

中文翻译：

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黑腹果蝇眼中蛋白质的绝对定量。

蛋白质的绝对（摩尔）定量决定了它们在复合物、网络和代谢途径中的摩尔比。MS Western 工作流程用于确定对黑腹果蝇眼中的形态发生和光转导 (PT) 可能至关重要的蛋白质的摩尔丰度，使用单个嵌合 264 kDa 蛋白质标准，总共涵盖 43 种蛋白质的 197 个肽。大多数蛋白质用 2 到 4 种蛋白型肽独立定量，变异系数小于 15%，动态范围优于 1000 倍，灵敏度为亚飞摩尔。在此，机械PT和rhabdomere，感光细胞器的蛋白质的摩尔丰度，在野生型的眼睛被确定飞行以及在屑（crb）突变的眼睛，表现出扰动的横纹肌形态发生。