Increasing evidence has shown that collagen peptides had various biological activities. In this study, a novel antiplatelet peptide Met‐Glu (ME) was separated and identified from silver carp skin by YMC ODS‐A C18 separation and ESI‐MS/MS analysis. Peptide ME inhibited platelet aggregation and secretion of platelet granules induced by ADP, thrombin and collagen, and significantly attenuated ferric chloride‐induced thrombus formation in rats. It did not prolong the bleeding time in mice even at the dose of 300 μmol/kg body weight that showed potent anti‐thrombosis effects. Additionally, peptide ME targeted at Gq‐protein to downregulate the phosphorylation of PLCβ, an important upstream effector of PI3K/Akt and Erk/MAPK signaling to inhibit intracellular calcium ion mobilization. These results suggest that peptide ME inhibited thrombosis in vivo and inhibited Gq‐mediated signaling in platelets, indicating the possibility that ME could potentially be developed as a novel therapeutic agent in the prevention and treatment of thrombotic diseases.

中文翻译：

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来自胶原蛋白水解物的新型二肽Met-Glu通过调节Gq介导的信号传导抑制血小板聚集和血栓形成。

越来越多的证据表明，胶原蛋白肽具有多种生物学活性。在这项研究中，通过YMC ODS-A C18分离和ESI-MS / MS分析从silver鱼皮肤中分离并鉴定了一种新型抗血小板肽Met-Glu（ME）。肽ME抑制了ADP，凝血酶和胶原蛋白诱导的血小板聚集和血小板颗粒的分泌，并显着减弱了氯化铁诱导的血栓形成。即使以300μmol/ kg体重的剂量表现出有效的抗血栓形成作用，它也不会延长小鼠的出血时间。此外，靶向ME的肽ME可以下调PLCβ的磷酸化，PLCβ是PI3K / Akt和Erk / MAPK信号传导的重要上游效应物，可抑制细胞内钙离子的动员。