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Vitamin C alleviates the senescence of periodontal ligament stem cells through inhibition of Notch3 during long-term culture.
Journal of Cellular Physiology ( IF 5.6 ) Pub Date : 2020-07-13 , DOI: 10.1002/jcp.29930
Yan Yang 1, 2, 3, 4 , Tao Wang 1, 2, 3, 4 , Sicheng Zhang 1, 2, 3, 4 , Sixun Jia 1, 2, 3, 4 , Hong Chen 1, 2, 3, 4 , Yufeng Duan 1, 2, 3, 4 , Shikai Wang 1, 2, 3, 4 , Guoqing Chen 2, 5 , Weidong Tian 1, 2, 3, 4
Affiliation  

Periodontal ligament stem cells (PDLSCs), as potential “seed cells” for periodontal tissue repair and regeneration, require to be expanded in vitro for a large scale. Senescence of PDLSCs occurred during long‐term culture may compromise the therapeutic effects of PDLSCs. Medium supplements may be useful in antisenescence. However, the effects and mechanisms of vitamin C (Vc) treatment on PDLSCs during long‐term culture are still unclear. In this study, we identified that Vc‐treated PDLSCs cells maintained a slender morphology, higher growth rate and migration capacity, stemness, and osteogenic differentiation capability during a long‐term culture. Moreover, we also identified that Notch3 was significantly upregulated during the cell senescence, and Vc treatment alleviated the senescence of PDLSCs through inhibition of Notch3 during long‐term culture. In summary, Vc treatment suppressed PDLSCs senescence by reducing the expression of Notch3 and might be a simple and useful strategy to inhibit cellular senescence during the cell long‐term culture.

中文翻译:

维生素 C 在长期培养过程中通过抑制 Notch3 来缓解牙周膜干细胞的衰老。

牙周膜干细胞(PDLSCs)作为牙周组织修复和再生的潜在“种子细胞”,需要在体外​​进行大规模扩增。在长期培养过程中发生的 PDLSCs 衰老可能会影响 PDLSCs 的治疗效果。培养基补充剂可能有助于抗衰老。然而,长期培养期间维生素 C (Vc) 治疗对 PDLSCs 的影响和机制尚不清楚。在这项研究中,我们发现 Vc 处理的 PDLSCs 细胞在长期培养过程中保持纤细的形态、更高的生长速率和迁移能力、干性和成骨分化能力。此外,我们还发现 Notch3 在细胞衰老过程中显着上调,Vc 处理通过在长期培养过程中抑制 Notch3 来缓解 PDLSCs 的衰老。总之,Vc 处理通过降低 Notch3 的表达来抑制 PDLSCs 衰老,并且可能是在细胞长期培养过程中抑制细胞衰老的一种简单而有用的策略。
更新日期:2020-07-13
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