A simple, accurate, and precise bioanalytical method was developed and validated for the determination of pharmacokinetic parameters of sodium copper chlorophyllin, a USFDA approved food additive and colorant in rat plasma. The column used was Luna® C18 250×4.6 mm, 100 Å, having particle size 4.5 μm, and the mobile phase used was methanol (MeOH), and 10 mM ammonium acetate buffer in the ratio of 90 : 10, the flow rate was 1 ml/min, and the injection volume of 20 μL. The retention time of sodium copper chlorophyllin was obtained at 9 min. The method was found to be linear at the range of 0.50-8.00 μg mL-1 .

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HPLC 法测定叶绿素铜钠 - 一种食品着色剂的开发和验证及其在药代动力学研究中的应用

开发并验证了一种简单、准确和精确的生物分析方法，用于测定叶绿素铜钠（一种美国食品药品监督管理局批准的食品添加剂和着色剂）在大鼠血浆中的药代动力学参数。所用色谱柱为Luna® C18 250×4.6 mm，100 Å，粒径4.5 μm，流动相为甲醇（MeOH），10 mM醋酸铵缓冲液，比例为90:10，流速为1 ml/min，进样量 20 μL。叶绿素铜钠的保留时间为 9 分钟。发现该方法在 0.50-8.00 μg mL-1 范围内呈线性。