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Platelet-rich plasma inhibits osteoblast apoptosis and actin cytoskeleton disruption induced by gingipains through upregulating integrin β1.
Cell Biology International ( IF 3.9 ) Pub Date : 2020-07-14 , DOI: 10.1002/cbin.11420 Weiyan Mo 1, 2 , Juan Wu 3 , Qihong Qiu 1, 4 , Fuping Zhang 1, 4 , Haoyuan Luo 1, 4 , Na Xu 5 , Wenjun Zhu 1, 4 , Min Liang 1, 4
Cell Biology International ( IF 3.9 ) Pub Date : 2020-07-14 , DOI: 10.1002/cbin.11420 Weiyan Mo 1, 2 , Juan Wu 3 , Qihong Qiu 1, 4 , Fuping Zhang 1, 4 , Haoyuan Luo 1, 4 , Na Xu 5 , Wenjun Zhu 1, 4 , Min Liang 1, 4
Affiliation
The aim of this study was to explore the effects of platelet‐rich plasma on gingipain‐caused changes in cell morphology and apoptosis of osteoblasts. Mouse osteoblasts MC3T3‐E1 cells were treated with gingipain extracts from Porphyromonas gingivalis in the presence or absence of platelet‐rich plasma. Apoptosis was detected with terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining. F‐actin was determined by phalloidin‐fluorescent staining and observed under confocal microscopy. Western blot analysis was used to detect integrin β1, F‐actin, and G‐actin protein expressions. A knocking down approach was used to determine the role of integrin β1. The platelet‐rich plasma protected osteoblasts from gingipain‐induced apoptosis in a dose‐dependent manner, accompanied by upregulation of integrin β1. Platelet‐rich plasma reversed the loss of F‐actin integrity and decrease of F‐actin/G‐actin ratio in osteoblasts in the presence of gingipains. By contrast, the effects of platelet‐rich plasma were abrogated by knockdown of integrin β1. The platelet‐rich plasma failed to reduce cell apoptosis and reorganize the cytoskeleton after knockdown of integrin β1. In conclusion, platelet‐rich plasma inhibits gingipain‐induced osteoblast apoptosis and actin cytoskeleton disruption by upregulating integrin β1 expression.
中文翻译:
富含血小板的血浆通过上调 β1 整合素抑制牙龈蛋白酶诱导的成骨细胞凋亡和肌动蛋白细胞骨架破坏。
本研究的目的是探讨富含血小板的血浆对牙龈蛋白酶引起的细胞形态变化和成骨细胞凋亡的影响。小鼠成骨细胞 MC3T3-E1 细胞用牙龈卟啉单胞菌的牙龈蛋白酶提取物处理 在存在或不存在富含血小板的血浆的情况下。用末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记染色检测细胞凋亡。F-肌动蛋白通过鬼笔环肽荧光染色确定并在共聚焦显微镜下观察。Western印迹分析用于检测β1整合素、F-肌动蛋白和G-肌动蛋白蛋白表达。使用敲低方法来确定 β1 整合素的作用。富含血小板的血浆以剂量依赖性方式保护成骨细胞免受牙龈蛋白酶诱导的细胞凋亡,同时上调 β1 整合素。在牙龈蛋白酶存在的情况下,富含血小板的血浆逆转了 F-肌动蛋白完整性的丧失和成骨细胞中 F-肌动蛋白/G-肌动蛋白比率的降低。相比之下,富含血小板的血浆的作用被 β1 整联蛋白的敲低所抵消。在敲低 β1 整合素后,富含血小板的血浆未能减少细胞凋亡和重组细胞骨架。总之,富含血小板的血浆通过上调整合素 β1 的表达来抑制牙龈蛋白酶诱导的成骨细胞凋亡和肌动蛋白细胞骨架的破坏。
更新日期:2020-09-15
中文翻译:
富含血小板的血浆通过上调 β1 整合素抑制牙龈蛋白酶诱导的成骨细胞凋亡和肌动蛋白细胞骨架破坏。
本研究的目的是探讨富含血小板的血浆对牙龈蛋白酶引起的细胞形态变化和成骨细胞凋亡的影响。小鼠成骨细胞 MC3T3-E1 细胞用牙龈卟啉单胞菌的牙龈蛋白酶提取物处理 在存在或不存在富含血小板的血浆的情况下。用末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记染色检测细胞凋亡。F-肌动蛋白通过鬼笔环肽荧光染色确定并在共聚焦显微镜下观察。Western印迹分析用于检测β1整合素、F-肌动蛋白和G-肌动蛋白蛋白表达。使用敲低方法来确定 β1 整合素的作用。富含血小板的血浆以剂量依赖性方式保护成骨细胞免受牙龈蛋白酶诱导的细胞凋亡,同时上调 β1 整合素。在牙龈蛋白酶存在的情况下,富含血小板的血浆逆转了 F-肌动蛋白完整性的丧失和成骨细胞中 F-肌动蛋白/G-肌动蛋白比率的降低。相比之下,富含血小板的血浆的作用被 β1 整联蛋白的敲低所抵消。在敲低 β1 整合素后,富含血小板的血浆未能减少细胞凋亡和重组细胞骨架。总之,富含血小板的血浆通过上调整合素 β1 的表达来抑制牙龈蛋白酶诱导的成骨细胞凋亡和肌动蛋白细胞骨架的破坏。