Equilibration time (ET) is the period during which sperm cells are in contact with cooling/freezing media components at a temperature of 5 °C, providing a proper osmotic balance between the intra- and extra-cellular milieu. The present study aimed to determine the ET (0, 2, and 4 h) that results in greater post-thaw sperm quality and functions. Based on the post-thaw sperm membrane integrity and motility ratios, 20 ejaculates collected from five boars were classified as having good (GFE, n = 5) or poor (PFE, n = 15) freezing capacity. Ratios of post-thaw sperm with intact plasma membrane and acrosome were similar between ET (0 h: 37.02 % ± 2.85 %; 2 h: 34.59 % ± 7.12 %; 4 h: 37.87 % ± 4.44 %) in GFE samples. In PFE, ratios of sperm with intact plasma membrane and acrosome at post-thaw were greater (P < 0.05) after an ET of 2 h than after an ET of 0 h (2 h: 26.16 % ± 1.54 % and 0 h: 16.74 % ± 1.59 %). Also, ratios of post-thaw sperm with relatively lesser membrane lipids disorder were greater (P < 0.05) after an ET of 2 h than for other ET in both GFE (2 h: 21.97 % ± 4.24 % and 0 h: 16.63 % ± 2.38 %) and PFE (2 h: 16.65 % ± 1.40 % and 0 h: 13.23 % ± 1.25 %) samples. In conclusion, an ET of 2 h results in greater sperm cryotolerance in both GFE and PFE samples, which suggests that modifying the freezing protocol lead to an increase post-thaw sperm function and survival.

平衡时间（ET）是精子细胞在5°C的温度下与冷却/冷冻培养基成分接触，以在细胞内和细胞外环境之间提供适当的渗透平衡的时期。本研究旨在确定可导致解冻后精子质量和功能增强的ET（0、2和4 h）。根据解冻后的精子膜完整性和运动率，从五个公猪中收集的20粒​​精液被分类为好（GFE，n  = 5）或差（PFE，n = 15）冷冻能力。在GFE样品中，ET之间的融化后精子与完整质膜和顶体的比率相似（0小时：37.02％±2.85％； 2小时：34.59％±7.12％； 4小时：37.87％±4.44％）。在PFE，具有完整细胞膜的精子的比例和顶体在后解冻是大于（P 比2小时后的ET后<0.05）0 h的ET（2小时：26.16％±1.54％和0 H：16.74 ％±1.59％）。同样，解冻后的精子与相对较少的膜脂紊乱的比率也更大（P ET 2小时后的GFE（2 h：21.97％±4.24％和0 h：16.63％±2.38％）和PFE（2 h：16.65％±1.40％和0 h： 13.23％±1.25％）样品。总之，ET 2 h会导致GFE和PFE样品中的精子耐低温性更高，这表明修改冷冻方案会导致解冻后精子功能和存活期增加。