An improved methodology of achieving an accurate separation of pure fractions of betacyanins and betaxanthins from Ullucus tuberosus Caldas has been carried out. For that purpose, an in-depth chemical identification of each betalain fraction using HPLC-DAD-ESI-MS was developed. This procedure allowed to evaluate the fractionation efficiency and also identify a large number of betalains, most of which have not been described so far in this raw material: betanidin- and isobetanidin-5-O-(4′-O-malonyl-β-glucoside), 2-decarboxy-phyllocactin, betanidin- and isobetanidin-6-O-(6′-O-feruloyl)-β-glucoside (gomphrenin and isogomphrenin III), dehydro-phyllocactin and isophyllocactin, and arginine and glycine-betaxanthins (portulacaxanthin III). Moreover, the availability of pure betalain fractions by the proposed methodology permitted to establish the total betalain content and the antioxidant activity of both separated betacyanin and betaxanthin fractions of ulluco for the first time. The results suggest Ullucus tuberosus as an underutilized food bioactive source with a high concentration of total betaxanthins (21.8 μg indicaxanthin/g fresh ulluco) and betacyanins (44.5 μg betanin/g fresh ulluco), strongly correlated to the Folin-Ciocalteau reduction capacity.

中文翻译：

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通过 HPLC-DAD-ESI-MS 从 Ulluco (Ullucus tuberosus Caldas) 分离的甜菜素和甜菜红素组分中鉴定新甜菜素

已经实施了一种改进的方法，可以从 Ullucus tuberosus Caldas 中准确分离纯组分甜菜红素和甜菜红素。为此，开发了使用 HPLC-DAD-ESI-MS 对每个甜菜碱级分进行深入化学鉴定的方法。该程序允许评估分馏效率并鉴定大量甜菜碱，其中大部分尚未在该原料中描述：甜菜碱-和异甜菜素-5-O-(4'-O-丙二酰-β- glucoside)、2-decarboxy-phyllocactin、betanidin-和isobetanidin-6-O-(6'-O-feruloyl)-β-glucoside（gomphrenin 和isogomphrenin III）、dehydro-phyllocactin 和isophyllocactin，以及精氨酸和甘氨酸-betaxanthins（马齿苋黄质 III)。而且，通过所提出的方法可以获得纯甜菜素级分，这使得首次能够确定 ulluco 分离的甜菜花青素和甜菜红素级分的总甜菜素含量和抗氧化活性。结果表明，Ullucus tuberosus 作为一种未充分利用的食物生物活性来源，具有高浓度的总甜菜黄素（21.8 μg 红黄素/g 新鲜 ulluco）和甜菜红素（44.5 μg 甜菜素/g 新鲜 ulluco），与 Folin-Ciocalteau 还原能力密切相关。