Oryza sativa (rice) is an economically important crop, which is valued as a staple food source and as genetic model for cereals. Rice production needs to increase substantially to meet the growing global demand. Genetic studies have indicated that anther and pollen development genes can be used as targets to improve yield. Although several genes related to male organ developmental pathways have been characterized, the complete signaling network remains unclear. We used publicly available anther and pollen development transcriptome datasets to identify candidates for functional genomics studies. In addition, we newly generated RNA-Seq data for anther, anther wall and pollen samples with leaf sheath as control to provide the expression data for genes that are not covered by microarray technologies in rice. We constructed a platform, the rice male gamete expression database (RMEDB), comprising microarray and RNA-Seq based 188 transcriptome samples across diverse rice tissues and developmental stages of O. sativa japonica and O. sativa indica. Meta-expression analysis identified genes expressed at specific stages of anther or pollen developments. The functionalities in RMEDB database (https://ricephylogenomics-khu.org/RMEDB/home.php) can serve to identify candidates in male organ development for functional genomic studies of rice.

水稻（稻米）是一种重要的经济作物，被视为主要食物来源和谷物的遗传模型。大米产量需要大幅度增加，以满足不断增长的全球需求。遗传研究表明，花药和花粉发育基因可以用作提高产量的靶标。尽管已鉴定了几种与雄性器官发育途径有关的基因，但完整的信号网络仍不清楚。我们使用了公众可获得的花药和花粉发育转录组数据集来确定功能基因组学研究的候选对象。此外，我们新生成了以叶鞘为对照的花药，花药壁和花粉样品的RNA-Seq数据，以提供水稻微阵列技术未涵盖的基因的表达数据。我们搭建了一个平台粳稻和in稻。元表达分析鉴定了在花药或花粉发育的特定阶段表达的基因。RMEDB数据库（https://ricephylogenomics-khu.org/RMEDB/home.php）中的功能可以用来确定水稻功能基因组研究中雄性器官发育的候选对象。