Solanum viarum Dunal is an important medicinal plant with a high quantity of steroidal alkaloids used for the synthesis of contraceptives, corticosteroids, and sex hormones. It is also used by Indian tribal people for the treatment of leprosy, toothache, and diabetes. Therefore, to meet the existing needs for this plant, it is necessary to develop an efficient regeneration system useful for rapid and large-scale clonal propagation with ensured genetic fidelity. An efficient and improved regeneration protocol for prickly and prickleless genotypes of S. viarum has been developed using three explants, leaf, petiole, and internodes, under the influence of two plant growth regulators, thidiazuron (TDZ) and 6-benzyladenine (BA). Effects of genotype, explant type, and concentrations of TDZ and BA were studied. A higher percentage of shoot organogenesis (78.25% ± 2.53) and shoot number per explant (6.79 ± 1.04) were achieved in the leaf segments of prickly genotype cultured on modified Murashige and Skoog (MS) medium supplemented with TDZ (1.50 mg L⁻¹). Furthermore, basal leaf segments showed 100% regeneration from the prickly genotype. A significantly higher content of total phenolics was quantified in prickleless (3.66 μg mg⁻¹) than prickly genotypes (2.73 μg mg⁻¹). The monomorphic banding pattern of random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) analysis confirmed the genetic fidelity of the regenerated plants. Additionally, flow cytometric analysis of regenerants showed no variation in the ploidy levels when compared to the mother (control) plants. These results clearly depicted the efficiency of developed protocol that can be utilized for generating genetically stable population of S. viarum.

龙葵（Solanum viarum Dunal）是重要的药用植物，其中含有大量的甾体生物碱，可用于合成避孕药具，皮质类固醇和性激素。印度部落居民还使用它来治疗麻风病，牙痛和糖尿病。因此，为了满足该植物的现有需求，有必要开发一种有效的再生系统，该系统可用于具有确保的遗传保真度的快速和大规模的克隆繁殖。一个有效和改进的再生协议，用于刺穿和无刺的S. viarum基因型。在两种植物生长调节剂，噻唑隆（TDZ）和6-苄基腺嘌呤（BA）的影响下，已使用三种外植体（叶，叶柄和节间）开发了大豆。研究了基因型，外植体类型以及TDZ和BA浓度的影响。在改良的Murashige和Skoog（MS）培养基中添加TDZ（1.50 mg L ^-1）培养的多刺基因型叶节中，获得更高的芽器官发生率（78.25％±2.53）和每个外植体的芽数（6.79±1.04）。）。此外，基生叶片段显示出多刺基因型的100％再生。总酚甲显著较高含量在prickleless（3.66微克毫克定量^-1）比刺基因型（2.73微克毫克^-1）。随机扩增多态性DNA（RAPD）和简单序列重复（SSR）分析的单态性谱带模式证实了再生植物的遗传保真度。另外，与母体（对照）植物相比，再生剂的流式细胞术分析显示倍性水平没有变化。这些结果清楚地描述了开发的协议的效率，可用于产生遗传稳定的S. viarum种群。