Applying a desirable disinfestation process is necessary to control the pathogenic microorganisms in the swimming pools and prevent both dermal and intestinal effects. Therefore, the present study was conducted to compare the bacterial community and diversity in the two swimming pools disinfected by the chlorine and ozone (O₃)-chlorine processes. A total of 24 samples were taken from the two swimming pools in three distinct seasons to analyze the bacterial and physico-chemical indicators. Culture and molecular methods were used to evaluate the microbial quality. Two sets of sample taken from the pools with the maximum swimmer load in the summer were investigated by the next-generation sequencing (NGS) technique. In total, 410 and 406 bacterial species were identified in the chlorine- and ozone-chlorine-disinfected pools, respectively. Among the eight dominant bacterial species in each swimming pool, Pseudomonas alcaliphila, Pseudomonas stutzeri, and Pseudomonas acnes were common species between the two studied pools. Oleomonas sagaranensis (350 reads/18593), Staphylococcus caprae (302 reads /18593), and Anaerococcus octavius (110 reads/18593) were among the dominant bacteria in the chlorine-disinfected pool. Bacterial diversity was lower in the ozone-chlorine-disinfected pool than the other one, and the highest bacterial sequencing belonged to the genus Pseudomonas (85.79%). Results showed that water quality of in O₃-chlorine-disinfected pool was more desirable than the chlorine-disinfected pool. Molecular methods along with conventional culture methods would be advantageous for microbial assessment in the swimming pools.

中文翻译：

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伊朗大不里士用臭氧-氯气和氯气工艺消毒过的游泳池中微生物质量的比较研究。

为了控制游泳池中的病原微生物并防止皮肤和肠道的影响，必须采用理想的消毒方法。因此，本研究的目的是比较两个被氯和臭氧（O ₃消毒）的游泳池的细菌群落和多样性。）-氯工艺。在三个不同的季节从两个游泳池总共采集了24个样本，以分析细菌和理化指标。培养和分子方法用于评估微生物质量。通过下一代测序（NGS）技术研究了夏天从游泳者最大的游泳池中采集的两组样本。总共在氯和臭氧-氯消毒池中分别鉴定了410和406种细菌。在每个游泳池的八大优势菌种，假单胞菌alcaliphila，施氏假单胞菌和假单胞菌痤疮是两个研究的池之间常见的品种。佐贺油麻（350读/ 18593），葡萄球菌（302读/ 18593）和八叶厌氧球菌（110读/ 18593）是氯消毒池中的主要细菌。臭氧-氯消毒池中的细菌多样性比另一种低，细菌最高的测序属于假单胞菌属（85.79％）。结果表明，与氯消毒池相比，O _3-氯消毒池的水质更为理想。分子方法与常规培养方法一起对于游泳池中的微生物评估将是有利的。