Abstract

Objectives

Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population.

Methods

Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followed by RT-qPCR validation of their expression in serum of 20 patients with lung cancer, 10 patients with COPD and 10 healthy volunteers. we examined their expression in 10 bronchoalveolar lavage samples and assessed correlation with the serum levels.

Results

There was an inverse relationship between expression of serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 (r = −0.62, p = .00). Both serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 showed a significant positive and negative association with lung cancer patients respectively in comparison to patients with COPD and healthy persons (p < .001).

Conclusion

RAB27A mRNA and Lnc-RNA-RP11-510M2.10 could be used as diagnostic and prognostic biomarker tools for lung cancer.

中文翻译：

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RAB27A及其相关lncRNA Lnc-RNA-RP11-510M2在肺癌中的外泌体表达

摘要

目标

检查血清外泌体 RAB27A mRNA 在肺癌中的诊断作用，并评估 LncRNA 与肺癌与埃及人群中 RAB27A mRNA 的关系。

方法

基于生物信息学方法选择基于外泌体 RNA 的生物标志物 RAB27A mRNA 和 Lnc-RNA-RP11-510M2.10，然后通过 RT-qPCR 验证它们在 20 名肺癌患者、10 名 COPD 患者和 10 名健康志愿者的血清中的表达. 我们检查了它们在 10 个支气管肺泡灌洗液样本中的表达，并评估了与血清水平的相关性。

结果

血清外泌体 RAB27A mRNA 的表达与 Lnc-RNA-RP11-510M2.10 呈负相关（r = -0.62，p  = .00）。与 COPD 患者和健康人相比，血清外泌体 RAB27A mRNA 和 Lnc-RNA-RP11-510M2.10 分别与肺癌患者呈显着正相关和负相关（p  < .001）。

结论

RAB27A mRNA 和 Lnc-RNA-RP11-510M2.10 可用作肺癌的诊断和预后生物标志物工具。