ABSTRACT

Diabetes mellitus (DM) is a common metabolic disorder with complications including nephropathy, cardiomyopathy, neuropathy and infertility. We investigated nitric oxide synthase (NOS) expression in uterine tissue of diabetic rats. The rats were assigned randomly to four groups of ten: group C, control; group 1, diabetic for 1 month; group 2, diabetic for 2 months; group 3, diabetic for 3 months. DM was induced by streptozotocin injection. nNOS, iNOS, eNOS expressions and TUNEL staining were assessed in uterine tissue and luteinizing hormone (LH), follicle stimulating hormone (FSH) and estrogen were measured in blood samples. The uterine epithelium, connective tissue of the endometrium, myometrium wall thickness and number of apoptotic cells were decreased in groups 1 − 3 compared to group C. nNOS and eNOS expression was increased in diabetic groups, but iNOS expression was similar to group 1. FSH was increased in group 2, but serum LH and estrogen levels were unchanged among groups. DM damages uterine tissue, and NOS, especially nNOS and eNOS, contributes to this damage.

摘要

糖尿病（DM）是一种常见的代谢性疾病，其并发症包括肾病、心肌病、神经病和不孕症。我们研究了糖尿病大鼠子宫组织中一氧化氮合酶 (NOS) 的表达。将大鼠随机分配到四组，每组十只：C 组，对照组；第1组，糖尿病1个月；第2组，糖尿病2个月；第3组，糖尿病3个月。DM由链脲佐菌素注射诱导。在子宫组织中评估了 nNOS、iNOS、eNOS 表达和 TUNEL 染色，并在血液样本中测量了促黄体激素 (LH)、促卵泡激素 (FSH) 和雌激素。与C组相比，1-3组的子宫上皮、子宫内膜结缔组织、子宫肌层壁厚度和凋亡细胞数量减少。糖尿病组nNOS和eNOS表达升高，但iNOS表达与1组相似。2组FSH升高，但各组血清LH和雌激素水平无变化。DM 会损害子宫组织，而 NOS，尤其是 nNOS 和 eNOS，会导致这种损害。