The state of the antioxidative defense system in the hepatopancreas and central nerve ganglia was investigated in the mollusk Lymnaea stagnalis in the normal conditions and one day after acute experimental hyperglycemia induced by a 2-h incubation in 100 mM glucose solution. It was established that hyperglycemic exposure caused a 1.6- and 1.5-fold increase in the reduced glutathione (GSH) level and superoxide dismutase (SOD) activity, respectively, as observed against the background of a 1.3-fold decrease in the total protein level. At the same time, activity of Se-dependent glutathione peroxidase (Se-GP) and the level of TBA reactive substances (TBARS) remained intact. Hepatopancreatic tissues were characterized by a 1.3-fold decrease in TBARS, as detected against the background of a 1.1-fold increase in the total protein level, while other antioxidative defense components (SOD, GSH, Se-GP) remained invariable. It is assumed that the more developed antioxidative system in central ganglia is essential for providing sustained functioning of nerve cells in L. stagnalis under dramatic disturbances of glucose homeostasis in the internal environment.

中文翻译：

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急性实验性高血糖后软体动物 Lymnaea stagnalis 肝胰腺和神经节的抗氧化防御

在正常条件下和通过在 100 mM 葡萄糖溶液中孵育 2 小时诱导的急性实验性高血糖症后一天，在软体动物 Lymnaea stagnalis 中研究了肝胰腺和中枢神经节中抗氧化防御系统的状态。在总蛋白质水平降低 1.3 倍的背景下观察到，高血糖暴露导致还原型谷胱甘肽 (GSH) 水平和超氧化物歧化酶 (SOD) 活性分别增加 1.6 倍和 1.5 倍。同时，硒依赖性谷胱甘肽过氧化物酶 (Se-GP) 的活性和 TBA 反应性物质 (TBARS) 的水平保持不变。在总蛋白水平增加 1.1 倍的背景下检测到，肝胰腺组织的特征是 TBARS 减少 1.3 倍，而其他抗氧化防御成分（SOD、GSH、Se-GP）保持不变。假设中央神经节中更发达的抗氧化系统对于在内部环境中葡萄糖稳态的显着干扰下提供 L. stagnalis 中神经细胞的持续功能是必不可少的。