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Salinity stress alters ion homeostasis, antioxidant activities and the production of rosmarinic acid, luteolin and apigenin in Dracocephalum kotschyi Boiss
Biologia ( IF 1.5 ) Pub Date : 2020-07-13 , DOI: 10.2478/s11756-020-00562-3
Farinaz Vafadar , Rayhaneh Amooaghaie , Parviz Ehsanzadeh , Mustafa Ghanadian

Salt stress affects growth and secondary metabolism in medicinal plants. There is no information about the impacts of salt stress on Dracocephalum kotschyi Boiss. Therefore, in this study, the 60-day old seedlings grown in coco peat and perlite were irrigated with various concentrations of NaCl (0, 25, 50, 75, and 100 mM) in accordance with a randomized complete block design for 3 weeks. The results showed that with increasing salt concentration, Na+ concentration was increased, but Ca2+ and K+ concentrations and fresh and dry weights were decreased in both roots and shoots. Besides, photosynthetic pigments and relative water content declined, whereas proline concentration and electrolyte leakage levels elevated in leaves under salinity stress. The activities of ascorbate peroxidase and guaiacol peroxidase enzymes were enhanced at lower salt concentrations but impaired under higher concentrations of NaCl. High-performance liquid chromatography showed that the contents of rosmarinic acid, luteolin, and apigenin were increased with aggravation of salt stress. The highest total phenolic and flavonoid contents and the rosmarinic acid, luteolin, and apigenin contents were verified at 75 mM NaCl that was concomitant with the most 2,2-diphenylpicrylhydrazyl scavenging activity in the leaves. In contrast, all these attributes decreased under 100 mM NaCl. In conclusion, the results unveiled that D. kotschyi is a moderate salt-tolerant plant and adaptive mechanisms adopted by this plant are including Na+ exclusion in roots, the activation of the antioxidant system, and the alteration of phenolic profile. This study also suggests that mild to moderate salinity can be employed as an elicitor to increasing some pharmaceutical important phenolic compounds in D. kotschyi.



中文翻译:

盐胁迫改变了Dracocephalum kotschyi Boiss的离子稳态,抗氧化活性以及迷迭香酸,木犀草素和芹菜素的产生

盐胁迫影响药用植物的生长和次级代谢。没有有关盐胁迫对Dracocephalum kotschyi Boiss的影响的信息。因此,在这项研究中,按照随机完整块设计,用不同浓度的NaCl(0、25、50、75和100 mM)灌溉在可可泥炭和珍珠岩中生长的60天龄幼苗3周。结果表明,随着盐浓度的增加,Na +浓度增加,而Ca 2+和K +根和茎中的浓度,鲜重和干重均降低。此外,盐分胁迫下叶片的光合色素和相对含水量下降,而脯氨酸浓度和电解质渗漏水平升高。在较低的盐浓度下,抗坏血酸过氧化物酶和愈创木酚过氧化物酶的活性增强,但在较高的NaCl浓度下,其活性减弱。高效液相色谱显示,随着盐胁迫的加剧,迷迭香酸,木犀草素和芹菜素的含量增加。在75 mM NaCl上验证了最高的总酚和类黄酮含量以及迷迭香酸,木犀草素和芹菜素含量,这与叶片中最大的2,2-二苯基吡啶并肼基清除活性相伴。相反,在100 mM NaCl下,所有这些属性均降低。总之,结果表明D. kotschyi是中等耐盐性植物,该植物采用的适应性机制包括根中Na +的排除,抗氧化剂系统的活化以及酚类成分的改变。这项研究还表明,轻度至中度盐度可以用作引发剂来增加科氏梭菌中某些重要的酚类化合物

更新日期:2020-07-13
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