Gardenia gummifera L.f. (Family: Rubeacea) is used in indigenous system of medicine to cure many diseases. To authenticate the traditional medicinal claim investigation has been under taken to evaluate the antioxidant and hepatoprotective activities of Gardenia gummifera L.f. fruit methanol extract (GFME). GFME was evaluated using various antioxidant assays, including DPPH and Nitric oxide radical scavenging assays. The protective effects of GFME were studied in carbon tetrachloride reduced biochemical markers of hepatic injury such as serum glutamyl oxaloacetate transaminase (SGOT), serum glutamyl pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB) and direct bilirubin (DB) and in silico studies were carried out to screen the GFME phytocompounds. The extract showed significant antioxidant activity in DPPH and Nitric oxide radical scavenging with IC50 value of 131.11 and 175.95 respectively. Quantitative phytochemical assay determines the presence of alkaloids 69.1 μg/1 mg and phenolics 76.5 μg/1 mg. GC-MS analysis of aromatic extract resulted in 36 compounds. Among them, compounds 2, 3-Dihydro-3,5-dihydroxy-6-methyl-4 h-pyran-4-one, 2-furancarboxaldehyde 5-(hydroxymethyl) and Quinic acid are the major ones. The fruit methanol extract showed significant in vivo hepatoprotective activity by altering the levels of liver function biochemical parameters such as SGOT, SGPT, ALP, TP, TB and DB. Histology of the liver section also confirms the hepatoprotective activity of GFME. Molecular docking of GC-MS profiled phytocompounds with the target protein TGF-β1and PPARα also confirmed the therapeutic effect with good hydrogen bonding and hydrophobic interactions. Thus the present study clearly strengthened the traditional medicinal claim of the plant Gardenia gummifera L.f. possessing the hepatoprotective drug.

中文翻译：

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抗氧化剂和体内的保肝作用栀子gummifera Lf的果实甲醇提取物

ia子gum Lf（家庭：Rubeacea）在本地医学系统中用于治疗许多疾病。为了验证传统的药物要求，已经进行了研究以评估of子Lf水果甲醇提取物（GFME）的抗氧化和保肝活性。使用各种抗氧化剂测定法（包括DPPH和一氧化氮自由基清除测定法）评估GFME。在降低四氯化碳对肝损伤的生化指标，例如血清谷氨酰草酰乙酸转氨酶（SGOT），血清谷氨酰丙酮酸转氨酶（SGPT），碱性磷酸酶（ALP），总蛋白（TP），总胆红素（TB）中，研究了GFME的保护作用。 ），直接胆红素（DB）和计算机模拟研究进行了筛选，以筛选GFME植物化合物。该提取物在DPPH和一氧化氮自由基清除中表现出显着的抗氧化活性，IC50值分别为131.11和175.95。定量植物化学测定确定存在69.1μg/ 1 mg生物碱和76.5μg/ 1 mg酚类化合物。芳烃萃取物的GC-MS分析结果为36种化合物。其中，化合物2、3-二氢-3,5-二羟基-6-甲基-4h-吡喃-4-酮，2-呋喃甲醛5-（羟甲基）和奎尼酸是主要化合物。水果甲醇提取物通过改变肝脏功能生化参数（例如SGOT，SGPT，ALP，TP，TB和DB）的水平，显示出显着的体内保肝活性。肝脏切片的组织学也证实了GFME的保肝活性。GC-MS分析的植物化合物与目标蛋白TGF-β1和PPARα的分子对接也证实了具有良好氢键和疏水相互作用的治疗效果。因此，本研究明显加强了具有保肝药的gum子植物Lf的传统医学主张。